background: in megavoltage radiotherapy rooms, ordinary concrete is usually used due to its low construction costs, although higher density concrete are sometimes used, as well. the use of high-density concrete decreases the required thickness of the concrete barrier hence, its disadvantage is its high cost. in a nuclear reactor, neutron radiation is the most difficult to shield. a method for p...

A microarray analysis was performed to investigate whether ex vivo culture conditions affect the characteristics of MSCs. Gene expression profiles were mainly influenced by the level of cell confluence rather than initial seeding density. The analysis showed that 276 genes were upregulated and 230 genes downregulated in MSCs harvested at ~90% versus ~50% confluence (P < 0.05, FC > 2). The genes...

The primary culture of neuronal cells plays an important role in neuroscience. There has long been a need for methods enabling the long-term culture of primary neurons at low density, in defined serum-free medium. However, the lower the cell density, the more difficult it is to maintain the cells in culture. Therefore, we aimed to develop a method for long-term culture of neurons at low density...

A predictive and feedback glucose feed controller, previously developed for nutrient-sufficient growth of Escherichia coli to high cell densities, was used to produce large quantities of a heterologous, cyanobacterial recombinant hexadecameric (L8S8) protein, ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) in E. coli. Culture and plasmid stability conditions were optimized to yield th...

In the studies reported here, we demonstrate that transforming growth factor alpha (TGF-alpha) or epidermal growth factor (EGF) is required for the establishment of small colonies of human keratinocytes at clonal densities, but once small (10-15 cells) colonies have formed, the continued growth of these colonies can proceed in the absence of exogenous TGF-alpha or EGF. Equivalent receptor-bindi...

background: chlamydia trachomatis is the most reported bacterial sexually transmitted disease, especially among young women worldwide. aim of this study was comparison to evaluate the prevalence of chlamydia trachomatis infection in woman with tubal infertility by means of pcr and cell culture techniques. methods: fifty-one women with confirmed tfi were enrolled in this study in (avicenna infer...

Microencapsulation technology is an alternative large-scale mammalian cell culture method. The semi-permeable membrane of the microcapsule allows free diffusion of nutrients, oxygen and toxic metabolites to support cell growth, and the microcapsule membrane can protect the cells from the mechanical damage of shear forces associated with agitation and aeration. Many polymers have been used to ma...

In this study, the effects of oxygen supplementation in different stocking densities of rainbow trout (Oncorhynchus mykiss) on the specific growth rates (SGR), feed conversion ratios (FCR) and survival rates were investigated in a commercial-scale culture system. High density cultures were maintained in a total of nine (12 m3) flow-through systems supplemented with O2. Initial stocking rates fo...

Recombinant alkaline polygalacturonate lyase (PGL) production by recombinant Pichia pastoris GS115 was selected as a model to study as a continuous culture strategy for enhancing heterologous protein production based on controlling methanol feeding (CCCM culture) or on dual carbon source feeding (CCCD culture). Using the CCCM process with a dry cell weight of 75 g/L regulated by controlling met...