An actin ruler n page 947, McElhinny et al. suggest that nebulin is a molecular ruler for actin filaments in muscle cells. Muscle physiology depends on the precise alignment, length, and overlap of thin (actin) and thick (myosin) filaments. Capping proteins such as Tmod stop the growing and shrinking of dynamic thin filaments but lack the innate ability to know when to do so. The new results sh...

two of the most widely cited models in biodiversity research; niche partitioning and adaptive radiation. Many Amazonian fish genera are represented by 10 or more species living together — cheek by jowl — in multi-species assemblages. Even the specialist can hardly tell them apart; they often have very similar shapes and sizes, diets and microhabitats. In the face of this exuberance arguments fo...

Reaction-diffusion systems produce a variety of patterns such as spots, labyrinths, and rotating spirals. Circular spots may be stationary or unstable to oscillating motion. The oscillations are sometimes steady but may lead to collapsing or infinitely expanding spots. Using a singular perturbation technique we derive a set of ordinary differential equations for the dynamics of circular spots. ...

Mouse lymphocyte H-2 and Ia glycoproteins have been analyzed with a two-dimensional (2-D) acrylamide gel electrophoresis technique, in which proteins are separated first according to their charge in isoelectrofocusing gels and then according to their size in sodium dodecyl sulfate gels. Individual polypeptide chains from radiolabeled cells are resolved as discrete spots on autoradiograms of the...

In the search for new markers of human endometrial hyperplasia and adenocarcinoma the method of quantitative two-dimensional gel electrophoresis was applied to study the protein expression profiles of metabolically [(35)S]-methionine-labelled proteins of endometrial explants. Approximately 1700 protein spots were resolved by the two-dimensional gel electrophoresis, and the expression pattern of...

Tropomyosin-related kinase A (TrkA) is a receptor-type protein tyrosine kinase and exploits pleiotypic roles via nerve growth factor (NGF)-dependent or NGF-independent mechanisms in various cell types. Here, we showed that the inhibition of TrkA activity by GW441756 resulted in the suppression of tyrosine phosphorylation of cellular proteins including extracellular signal-regulated protein kina...

Identification of biomarkers could lead to the development of effective screening tests for colorectal cancer. A previous study from our laboratory showed specific alterations of nuclear structure in colon cancer. In an effort to characterize these biomarkers, protein spots were selected from separations made by two-dimensional gel electrophoresis, which were analyzed by mass spectrometry. The ...

In this Letter, we introduce conical-scanning dark-field imaging in four-dimensional (4D) ultrafast electron microscopy to visualize single-particle dynamics of a polycrystalline ensemble undergoing phase transitions. Specifically, the ultrafast metal-insulator phase transition of vanadium dioxide is induced using laser excitation and followed by taking electron-pulsed, time-resolved images and...

Two new species, Hypancistrus phantasma and Hypancistrus margaritatus, are described based on material from the Rio Negro drainage. Both species are distinguished from congeners by unique color patterns. Hypancistrus phantasma is described from the Rio Uaupes and differs from congeners by having a tan body with small dark spots (vs. dark with light spots or with saddles or stripes). Hypancistru...

Urothelial bladder carcinoma (UBC) is heterogeneous in its pathology and clinical behaviour. Evaluation of prognostic and predictive biomarkers is necessary, in order to produce personalised treatment options. The present study used immunohistochemistry to evaluate UBC sections containing tumour and non-tumour areas from 76 patients, for the detection of p-mTOR, CD31 and D2-40 (blood and lympha...