sugar beet roots and attached soil are usually transferred to sugar factories for processing ,then the roots are washed by pressurized water. muddy water as waste flows in canal and is used to irrigate fields. the possibility of occurrence and fluctuations of the pathogenic organisms in waste were studied in 2005. the samples were collected from waste water once a week. isolation of pathogens w...

A key based on cyst and juvenile characters is given for identification of 12 valid Heterodera species in the H. avenae group. A compendium providing the most important diagnostic characters for use in identification of species is included as a supplement to the key. Cyst characters are most useful for separating species; these include shape, color, cyst wall pattern, fenestration, vulval slit ...

Using a scanning electron microscope and an interference contrast microscope, distinct morphological differences were found in the terminal areas of white females and cysts of six species of the genus Heterodera (s.g. Globodera). Suffident differences to separate species were established on the basis of length of vulval aperture in the white female; arrangement, number and size of the perineal ...

A simple, rapid, and inexpensive method for evaluation of host-parasite interactions, based on monoxenic cultures, is described. Axenic root explants of Glycine max (L.) Merr., cultured on a holidic agar medium, were inoculated with axenic second-stage larvae of Heterodera glycines Ichinohe, Race 3. A clear separation of susceptible and resistant cultivars, based on numbers of mature female nem...

Laser scanning confocal microscopy (LSCM) was used to localize the reactivity of a monoclonal antibody (Sv2) that binds to the subventral pharyngeal glands of preparasitic juveniles of Heterodera glycines. The greater resolution, magnification, and image analysis of LSCM compared with conventional epifluorescent microscopy enabled Sv2 binding to be localized much more precisely to the periphery...

A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic ne...