The generation of complement-dependent chemotactic activity was demonstrated by herpes simplex virus (HSV) antigen, HSV antigen-antiviral antibody complexes, and HVS-infected cell lysates using the Boyden in vitro assay. In the presence of normal serum, chemotactic activity was generated by lysates of uninfected cells when nonspecific cell damage released chemotactic intracellular proteolytic e...

Ocular herpes simplex virus (HSV) infection remains a major cause of corneal blindness. Several topical and oral antiviral medications have been used to treat herpetic keratitis. Advances in topical ophthalmic antivirals have been made over the past several decades. The first antivirals that were discovered were cytotoxic, while the antivirals developed more recently, such as acyclovir and ganc...

Thymidine kinase (TK) activity was examined in extracts of TK-deficient cells infected with low passage isolates of herpes simplex virus type 1 (HSV-1) obtained from patients with herpetic keratitis. TK activity induced by the virus, relative phosphorylation rates of thymidine and iododeoxyuridine (IUDR), and Km values for thymidine and for IUDR were compared for TK induced by the different vir...

In qualitative experiments, virus was recovered as regularly from herpes simplex virus-infected rabbits receiving IUDR as from control rabbits. In a quantitative experiment performed on a few rabbits, virus was recovered in the same titer from lUDR-treated rabbits as from control rabbits for the first 2 days of therapy. Not until the third day was a lower titer observed in the IUDR-treated rabb...

more potent drugs have become available. Arabinoside adenine and trifluorothymidine (TFT) are both effective and relatively nontoxic alternatives widely used in the management of herpetic disease.' Acyclovir (ACV) (9-(2-hydroxyethoxymethyl) guanine)2 and (E)-5(2-bromovinyl)-2' deoxyuridine),3 recently developed antiviral compounds with potent inhibitory activity against herpes simplex DNA polym...

We studied the influence of lgh-linked genes on the development of keratopathy after corneal inoculation with herpes simplex virus (HSV). Using congenic strains of mice, we found that the lgh-1 gene locus, or genes closely linked to it, influence the clinical expression of HSV infection. Mice with the lgh-1e or lgh-1d allotype routinely developed severe keratopathy after HSV corneal inoculation...

Famciclovir (FCV) is efficacious in the treatment of acute herpes zoster and recurrent genital infections but has not been used to treat ocular herpes simplex virus (HSV) infections. We evaluated the efficacy of orally administered FCV in treating HSV-1 epithelial keratitis and determined its effects on the establishment of latency and subsequent reactivation. Rabbits were inoculated with HSV-1...

Scanning electron microscopy of the endothelium of experimental disciform keratitis revealed corneal endothelial changes which distinguished disciform oedema from the more progressive stages of disciform keratitis. The endothelium of corneas with disciform oedema were wholly intact and characterised by subtle morphological alterations. In contrast, the more progressive stages of disciform kerat...

c.ontinuing recurrence of disease is one of the most troublesome characteristics of herpes simplex keratitis. If ocular herpes were a one-time infection, the likelihood of deep involvement, with its scarring, decreased vision, and even blindness, would be much smaller. With each recurrence, the chance of encountering these complications increases. Recent advances in research have led to a bette...

Herpes simplex keratitis (HSK) is a major cause of corneal blindness in the world. Following the primary infection, the virus enters into a latent phase. Recurrent infectious or immune keratitis cause structural damage to the cornea, scarring, and may lead to blindness. Several commercially available topical and oral antiviral drugs for HSK are currently available. However, toxicity and low pat...