The incorporation of L-[U-14C]lysine into the Q and /3 chains of hemoglobin has been compared in acetylphenylhydrazineinduced rabbit reticulocytes and normal and acetylphenylhydrazine-induced rabbit marrow. Evidence for a pool of Q chains and a/3 dimers in both reticulocytes and marrow cells was found in (a) an LY: /3 specific activity ratio of less than 1.0 in purified hemoglobin, (b) the rela...

BACKGROUND Severe anemia (hemoglobin < 70 g/L) in pregnancy may increase the risk of maternal and perinatal mortality. OBJECTIVES We assessed response to standard treatment with high-dose iron-folic acid for 90 d and single-dose (500 mg) mebendazole among severely anemic pregnant women in periurban Karachi, Pakistan. In addition, we evaluated the efficacy of 2 enhanced treatment regimens. D...

T HE HUMAN HEMOGLOBINS consist of two pairs of peptide chains.1’2 Adult hemoglobin ( Hb-A ) has two chains designated a and two chains designated iB; it is thus represented as 2A $2A#{149}Fetal hemoglobin (Hb-F ) has two a chains, which are similar if not identical in structure to those of Hb-A, but a pair of chemically distinct chains, the y chains replacing the $ chains.3’4 Hb-F is thus repre...

Globin messenger R N A ( m R N A ) levels in Fr iend v i rus t rans formed mouse cells have been es t imated by in situ hybr id iza t ion of D N A copy ( c D N A ) to fixed p repa ra t ions of cells and by hybr id iza t ion of c D N A to ext rac ted cy top lasmic R N A in true solution. The results obta ined by both methods agree in showing that a low level of globin m R N A can be detected in ...

Ascites syndrome (AS) is manifested in flocks of contemporary broilers that are allowed to fully manifest their genetic potential for rapid growth. After successful selection, a pair of divergent lines was established, AS-susceptible (AS-S) and AS-resistant (AS-R). These lines facilitate comparisons between genetically resistant and susceptible healthy young broilers when reared under standard ...

OBJECTIVE We investigated the impact of vitamin D supplementation on glucose metabolism in Vitamin D-deficient patients with prediabetes. METHODS A total of 66 subjects with the mean ages 52.2±9.9 years were included in this prospective and a 6-month follow-up study between 2008-2010. Vitamin D deficient patients (<25ng/ml) were supplemented with oral Vitamin D. RESULTS Vitamin D deficiency...

By D. L. RUCKNAGEL, E. B. PAGE AND W. N. JENSEN S TUDIES OF HEMOGLOBIN by the various technics of electrophoresis aud alkali denaturatiomi have resulted imi a strikimig development in the knowledge of abnormal hemoglobins amid in the hereditary hemolytic amieniias, some of them never previously recognized. During the course of the examination of the electrophoretic properties of the hemoglobimi...

BACKGROUND Previous evaluations of HPLC as a tool for detection of hemoglobin variants have done so within newborn-screening programs and/or by use of stored samples. We describe a 32-month prospective study in a clinical diagnostic laboratory in which we evaluated the imprecision of HPLC retention times and determined the retention times for hemoglobin variants seen in a multiethnic setting. ...

Deoxygenation-dependent association of hemoglobin tetramers appears to be widespread among amphibians, reptiles, and possibly all or most birds. The evidence for this conclusion depends largely on oxygen equilibria of whole blood which have Hill coefficients that reach values as high as 5-7 at 80-90% oxygenation. Computer simulation of the sedimentation velocity behavior of the major components...