Normal steady-state hematopoiesis takes place in the bone marrow microenvironment. Soluble factors as well as contact interactions between the hematopoietic cells and the marrow microenvironment dictate the fate of hematopoietic stem cells and progenitors. Over the last decade it has become clear that cell-cell and cell-extracellular matrix interactions through adhesion receptors play a major r...

Developing an integrative approach to early treatment response classification using survival modeling and bioinformatics with various biomarkers for assessment of filgrastim effects in amyotrophic lateral sclerosis patients. Filgrastim, a hematopoietic growth factor excellent safety, routinely applied oncology stem cell mobilization, had shown preliminary efficacy ALS.

purpose: to report the outcomes of conjunctival-limbal autograft (clau) in patients with unilateral total limbal stem cell deficiency (lscd) emphasizing surgical problems, complications, and their management. methods: in this prospective interventional case series, clau combined with amniotic membrane transplantation as a graft was performed on 26 patients with unilateral total lscd due to chem...

Every day, billions of new blood cells are produced in the body, each one derived from a hematopoietic stem cell (HSC). Because most mature blood stem cells have a limited life span, the ability of HSCs to perpetuate themselves through self-renewal and generate new blood cells for the lifetime of an organism is critical to sustaining life. A key problem in hematopoietic stem cell biology is how...

Relative quiescence is a defining characteristic of hematopoietic stem cells, while their progeny have dramatic proliferative ability and inexorably move toward terminal differentiation. The quiescence of stem cells has been conjectured to be of critical biologic importance in protecting the stem cell compartment, which we directly assessed using mice engineered to be deficient in the G1 checkp...

BACKGROUND Hematopoietic stem cells located in the bone marrow interact with a specific microenvironment referred to as the stem cell niche. Data derived from ex vivo co-culture systems using mesenchymal stromal cells as a feeder cell layer suggest that cell-to-cell contact has a significant impact on the expansion, migratory potential and 'stemness' of hematopoietic stem cells. Here we investi...

background: cell therapy has emerged as a promising curative intervention for several diseases including diabetes and wolfram syndrome (ws). the current study aimed to assess the effectiveness of clinical application of fetal-liver derived stem cells for treatment of patients with ws. methods: six patients with ws aged 23-34 (mean: 29.50, sd: 4.76) were recruited for the current phase 3 single-...

Cells in murine muscle have been reported to differentiate into hematopoietic stem and progenitor cells and thus repopulate the hematopoietic system of an irradiated animal. This activity was attributed to muscle stem cells. We used an in vitro and in vivo approach to identify the hematopoietic repopulating activity found in muscle tissue of mice by antibody staining and cell sorting. We confir...

cord blood is a rich source of hematopoietic stem cells and could be potentially used for transplantation instead of conventional sources of stem cells (bone marrow or peripheral blood). cord blood cells are successfully used in pediatric and adult patients, but their major limitation is the low number of hematopoietic stem cells for patients of large body size. there are several possible solut...