Although nonlinear microscopy and fast (approximately 1 ms) membrane potential (Vm) recording have proven valuable for neuroscience applications, their potentially powerful combination has not yet been shown for studies of Vm activity deep in intact tissue. We show that laser illumination of neurons in acute rat brain slices intracellularly filled with FM4-64 dye generates an intense second-har...

We have proposed an optical probe that can be used to characterize the orientation of collagen fibers in human dermis. A specific probing ability for collagen results from the use of second-harmonic-generation (SHG) light induced by collagen molecules in the tissue. Based on the concept of collagen SHG light, a reflection-type polarization measurement system (named SHG polarimetry) with a probe...

Three-dimensional second-harmonic fields, sample orientation, and susceptibility ratios of biological samples are measured using polarization-resolved second-harmonic generation (SHG) microscopy. The three-dimensional (3D) polarization is gathered by measurement of a series of holograms for which excitation and analyzer polarizations are systematically varied, and the 3D SHG field is recovered ...

This paper deals with the dependence of second order nonlinear susceptibility and second harmonic generation (SHG) efficiency on the shape of CdS quantum dot. A relation has been found between the electronic structure and second order nonlinear susceptibility. The effective mass approximation has been used to find the energy levels. We have considered both intra band and inter band near resonan...

In this work, a potential semiorganic nonlinear optical candidate NH4B[D-(+)-(C4H4O5)]2·H2O (NBC) has been studied using Density Functional Theory. The origin of the second harmonic generation (SHG) effect of NBC crystals for the NH4B[D-(+)-(C4H4O5)]2·H2O molecular complex is explained by employing a combination of the density of states, SHG density and molecular orbital analysis. It reveals a ...

Collinear third-harmonic generation at 526.7 nm was realized by the simultaneous phase matching of two second-order processes in a single quadratic crystal: second-harmonic generation (SHG) and sum-frequency mixing (SFM). The measured conversion efficiency was 12%. As a nonlinear medium a LiNbO(3) nonlinear photonic crystal with short-range order was used that allowed simultaneous phase matchin...

For a number of applications it is desirable to frequency double the output from high-power mode-locked ruby and neodymium lasers. Second harmonic generation (SHG) in LiNb03 and KHaPO. (KDP) crystals from picosecond light pulses emitted by a Nd3+:glass laser has previously been studied theoretically and experimentally. 1-3 This letter deSCribes details of an efficient intracavity second harmoni...

Based on cascading nonlinear interactions of second harmonic generation (SHG) and difference frequency generation (DFG), we present a novel scheme to control the group velocity of femtosecond pulse in MgO doped periodically poled lithium niobate crystal. Group velocity of tunable signal pulse can be controlled by another pump beam within a wide bandwidth of 180nm. Fractional advancement of 2.4 ...

OBJECTIVES This study aimed to evaluate the histological and mechanical features of tendon healing in a rabbit model with second-harmonic-generation (SHG) imaging and tensile testing. MATERIALS AND METHODS A total of eight male Japanese white rabbits were used for this study. The flexor digitorum tendons in their right leg were sharply transected, and then were repaired by intratendinous stit...