recombinant human interferon alpha 2a (rhifn α -2a) production and cell growth were monitored in a set of genetically modified e. coli strains (msd1519, msd1520, msd 1521, msd 1522, msd 1523) producing rhifn α -2a. the growth was followed at od 600 nm, changes in cell physiology were detected by pyrolysis mass spectrometry (pyms) of cell biomass and recombinant protein production was determined...

OBJECTIVE The purpose of this study was to develop alternative Strain Index risk classification categories. BACKGROUND Strain Index scores are usually categorized into four Strain Index "risk categories." The "original" risk categories were developed in the meat-packing industry and may not be fully applicable to other industries. METHOD Daily Strain Index scores were estimated among 276 ma...

Two field isolates of Rhizoctonia solani were isolated from infected paddy plants in Malaysia. These isolates were verified via ITS-rDNA analysis that yielded ~720 bp products of the ITS1-5.8S-ITS4 region, respectively. The sequenced products showed insertion and substitution incidences which may result in strain diversity and possible variation in disease severity. These strains showed some re...

CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were ...

This study assessed the accuracy of a cardiorespiratory monitoring device versus polysomnography for the diagnosis of suspected sleep apnoea/hypoponea syndrome (SAS). A total of 86 patients (89% male, mean age 52 yrs) that had been referred to a sleep laboratory with a clinical diagnosis of SAS underwent cardiorespiratory polygraphy in an unattended mode using an ambulatory device (MERLIN). Ana...

A digital recording device developed to monitor heart rate (HR) and breathing sounds (snoring), and used to screen subjects for obstructive sleep apnoea syndrome (OSAS), was investigated. This device is called the MESAM and is currently commercially available in some western European countries. The computer-based automatic scoring systems provided with the equipment and a hand-scoring technique...

We present a new context based event indexing and event ranking model for News Articles. The context event clusters formed from the UNL Graphs uses the modified scoring scheme for segmenting events which is followed by clustering of events. From the context clusters obtained three models are developed-Identification of Main and Sub events; Event Indexing and Event Ranking. Based on the properti...

BACKGROUND Heat stress as a physical harmful agent can increase the risk of health and safety problems in different workplaces such as mining. Although there are different indices to assess the heat stress imposed on workers, choosing the best index for a specific workplace is so important. Since various criteria affect an index applicability, extracting the most effective ones and determining ...

heat stress is one of the main obstacles for crop production in many areas of the world including southwest of iran. in order to evaluation the effect of heat stress on yield, yield component, and mopho-phenological traits of wheat, an experiment was conducted during 2010-2011 under normal (optimum sowing date) and heat stress (late sowing date) conditions on 11 wheat genotypes including yavaro...