Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease Cas9 has become a popular method for genome editing in several organisms. Most HDR protocols rely on plasmid-based expression of Cas9 and the gene-specific guide RNAs. Here we report that direct injection of in vitro-assembled Cas9-CRISPR RNA (crRNA) trans-activating crRNA (tracrRNA) ribonucleoprotein complexes into...

This work presents a framework for shading of virtual objects using high dynamic range (HDR) light probe sequences in real-time. The method is based on using HDR environment map of the scene which is captured in an on-line process by HDR video camera as light probes [32]. In each frame of the HDR video, an optimized CUDA kernel is used to project incident lighting into spherical harmonics in re...

In this paper, we present a computationally efficient, practically easy to use tone mapping techniques for the visualization of high dynamic range (HDR) images in low dynamic range (LDR) reproduction devices. The new method, termed hierarchical nonlinear linear (HNL) tone-mapping operator maps the pixels in two hierarchical steps. The first step allocates appropriate numbers of LDR display leve...

Hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) syndrome is an autosomal dominant condition caused by mutations of the gene encoding the dual zinc-finger transcription factor, GATA3. A previous study identified some patients with GATA3 gene variants and breast cancer, suggesting that GATA3 variants may contribute to tumorigenesis in estrogen receptor 1-positive breast t...

High-dose-rate intraoperative radiation therapy (HDR-IORT) has historically provided effective local control (LC) for patients with unresectable and recurrent tumors. However, IORT is limited to only a few specialized institutions and it can be difficult to initiate an HDR-IORT program. Herein, we provide a brief overview on how to initiate and implement an HDR-IORT program for a selected group...

Video recording and photography are frequently used to document Cultural Heritage (CH) objects and sites. High Dynamic Range (HDR) imaging is increasingly being used as it allows a wider range of light to be considered that most current technologies are unable to natively acquire and reproduce. HDR video content however has only recently become possible at desirable, high definition resolution ...

PURPOSE To report peri-operative fractionated high-dose-rate (HDR) brachytherapy with a 3D customized Freiburg flap applicator to treat locally recurrent Wilms' tumor, followed by immediate hyperthermic intraperitoneal chemotherapy for a 16-year-old with a second recurrence of nephroblastoma (Wilms' tumor). MATERIAL AND METHODS The tumor was excised and surgical bed was treated with fractiona...

This article describes the use of the parallel processing capabilities of a graphics chip to increase the processing speed of a high dynamic range (HDR) video system. The basis is an existing HDR video system that produces each frame from a sequence of regular images taken in quick succession under varying exposure settings. The image sequence is processed in a pipeline consisting of: shutter s...

As a part of routine oesophageal HDR Brachytherapy procedure, treatment planning takes about 45 minutes while patients are under light sedation. Some patients may suffer gagging and/or spasms before the 90-minute brachytherapy procedure complete, and the treatment may need to be aborted. A pre-prepared plan generated before patient’s sedation may reduce the brachytherapy procedure time by 40 mi...

A high dynamic range (HDR) image uses a large bit depth up to 32-bit per pixel per color channel. Due to hardware limitation, it can neither be captured by conventional camera in a single photo, nor be displayed on a conventional monitor. In this thesis, a software solution of HDR imaging is introduced from the synthesis to the display of high quality HDR images. Contrary to conventional imagin...