Sodium-bicarbonate cotransport in retinal glial cells was studied in the everted eyecup preparation of the rat. Intracellular pH was monitored with the indicator dye BCPCF and fluorescence confocal microscopy. Raising the K+ concentration from 3 to 12 mM in HCO3- -buffered perfusate evoked an intracellular alkalinization in both astrocytes and Müller cells. The alkalinization developed more rap...

We have developed a technique to measure the fluorescence of a pH-sensitive dye (2,7-biscarboxyethyl-5(6)-carboxyfluorescein) in single glomerular mesangial cells in culture. The intracellular fluorescence excitation ratio of the dye was calibrated using the nigericin-high-K+ approach. In the absence of CO2-HCO3-, mesangial cells that are acid loaded by an NH+4 prepulse exhibit a spontaneous in...

According to the hydrogeological zoning in western region of Mongolia, there are 9 hot springs such as Gantz mod, Chikhertei, Aksu, and Indert spring Mongol-Altai mountains, Otgontenger, Khojuul, Zart, Tsetsuuh, Ulaan Khaalga Khangai mountains. In this study, we selected these research objects. The aim was determine chemical composition mineral rocks dissolved springs, water-rock interaction, t...

We have used a sensitive new technique to assess the mechanism(s) of adrenergic inhibition of rainbow trout (Oncorhynchus mykiss) red blood cell (RBC) carbon dioxide excretion in vitro. The effect was only apparent using blood acidified to simulate metabolic acidosis. Red blood cell CO2 excretion was inhibited in a dose-dependent manner by physiologically relevant concentrations of noradrenalin...

The effect of dexamethasone on cytosolic pH (pHc) in resident mouse peritoneal macrophages was investigated using the fluorescent probe 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein tetra-acetoxymethyl ester (BCECF-AM). Dexamethasone was found to significantly lower pHc and this reduction of pHc evolved gradually with time, was near maximal at 10 nM dexamethasone, and could be prevented by th...

Bangong Lake is a narrow and long lake in the arid region of plateau northern Tibet. The salinity east different from that west, resulting differences natural environment human living conditions on each side. Watershed hydrochemical analysis spatial statistical can help to understand regional evolution water quality evaluation. In this study, characteristics surface (glacier, river, lake) groun...

Congenital chloride diarrhoea is an autosomal recessive disease characterized by life-long watery diarrhoea of prenatal onset with high faecal Cl- concentration. Seventy-nine patients have so far been reported. The basic defect involves the active Cl-/HCO3- exchange mechanism of the distal ileum and colon. The defect causes impaired absorption of Cl-, acidity of intestinal contents because of i...

The Isti Su hot spring is located in southeast of Salmas, in Western Azerbaijan, Iran. The area is a small part of Sanandaj-Sirjan geological zone which hosts many hot springs. The magma chamber of Salmas region is the main heat source of the hot springs in Isti Su area. Abundant rainfall and relatively long duration of snowfall as well as several deep faults in the area create the best conditi...

The stimulatory pathways controlling HCO3- secretion by the pancreatic ductal epithelium are well described. However, only a few data are available concerning inhibitory mechanisms, which may play an important role in the physiological control of the pancreas. The aim of this study was to investigate the cellular mechanism by which substance P (SP) inhibits pancreatic ductal HCO3- secretion. Sm...

AIM To investigate the effect of Hemp seed soft capsule (HSCC) on colonic ion transport and its related mechanisms in constipation rats. METHODS Sprague-Dawley male rats were randomly divided into three groups: normal group, constipation group and HSSC group. Rats in the constipation and HSSC groups were administrated loperamide 3 mg/kg per day orally for 12 d to induce the constipation model...