Sciortino, Christopher M., and Michael F. Romero. Cation and voltage dependence of rat kidney electrogenic Na-HCO3 2 cotransporter, rkNBC, expressed in oocytes. Am. J. Physiol. 277 (Renal Physiol. 46): F611–F623, 1999.— Recently, we reported the cloning and expression of the rat renal electrogenic Na-HCO3 2 cotransporter (rkNBC) in Xenopus oocytes [M. F. Romero, P. Fong, U. V. Berger, M. A. Hed...

An evaluation of several potential factors limiting carbon dioxide excretion by rainbow trout (Oncorhynchus mykiss) red blood cells was performed in vitro using a recently developed radioisotopic assay. Red blood cell (RBC) CO2 excretion was reduced by pretreatment (30min) of blood with the carbonic anhydrase inhibitor acetazolamide (final nominal concentration 1024 mol l21) or the Cl/HCO3 exch...

OBJECTIVE To compare pancreatic duct cell function in smokers (current and past) and never smokers by measurement of secretin-stimulated peak bicarbonate concentration ([HCO3-]) in endoscopic collected pancreatic fluid (PF). METHODS This retrospective study was cross-sectional in design, recording demographic information (age, gender, etc.), smoking status (former, current, never), alcohol in...

The study determines the major ion chemistry of Teesta River catchment in Eastern Himalayan region and evaluates hydrochemical processes controlling water composition area. Water basin was neutral to alkaline condition. HCO3−, Ca2+, Na+, Mg2+ SO42- were identified as dominant ionic species Ca-Mg-HCO3 facies. Higher contribution (Ca2++Mg2+) HCO3− towards TZ+ TZ−, high ratios (Ca2++Mg2+)/(Na++K+)...

The effects of capsaicin and mucosal acidification on gastric HCO3(-) secretion were compared in wild-type and prostacyclin (PGI2) IP receptor or prostaglandin E receptor EP1 or EP3 knockout C57BL/6 mice as well as rats. Under urethane anesthesia, the stomach was mounted on an ex vivo chamber, perfused with saline, and the secretion of HCO3(-) was measured at pH 7.0 using the pH-stat method. Ca...

Intracellular microelectrode techniques and intracellular pH (pHi) measurements using the fluorescent dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) were employed to characterize an electrogenic bicarbonate transport mechanism at the apical membrane of the frog retinal pigment epithelium (RPE). Reductions in apical concentrations of both [HCO3]o (at constant Pco2 or pHo) or [Na]o c...

The hypothesis of Cl-/HCO3- exchange across the apical membrane of the epithelial cells of Necturus gallbladder was tested by means of measurements of extracellular pH (pHo), intracellular pH (pHi), and Cl- activity (alpha Cli) with ion-sensitive microelectrodes. Luminal pH changes were measured after stopping mucosal superfusion with a solution of low buffering power. Under control conditions,...

Mechanisms that regulate endolymphatic pH are unknown. It has long been recognized that, because of the large positive endolymphatic potential in the cochlea, a passive movement of protons would be directed out of endolymph leading to endolymphatic alkalization. However, endolymphatic pH is close to that of blood, suggesting that H+ is being secreted into endolymph. Since the kidney and the inn...

33 High mobility group box 1 (HMGB1) is a damage-associated molecule implicated in 34 mediating kidney dysfunction in sepsis and sterile inflammatory disorders. HMGB1 is a nuclear 35 protein released extracellularly in response to infection or injury, where it interacts with TLR4 36 and other receptors to mediate inflammation. Previously we demonstrated that LPS inhibits 37 HCO3 absorption in t...