Dehalogenase-producing bacterium has been extensively studied due to their ability reduce pollution in the environment. A previous study reported that Bacillus megaterium WSH-002 contains a putative haloacid dehalogenase type II gene, therefore, presence of other genes associated with regulatory gene function and uptake halogenated compounds are expected. In whole genome, (DehWSH002) two relate...

Methylobacterium sp. strain DM4 and Methylophilus sp. strain DM11 can grow with dichloromethane (DCM) as the sole source of carbon and energy by virtue of homologous glutathione-dependent DCM dehalogenases with markedly different kinetic properties (the kcat values of the enzymes of these strains are 0.6 and 3.3 S-1, respectively, and the Km values are 9 and 59 microM, respectively). These stra...

The different steps of the dehalogenation reaction carried out by LinB on three different substrates have been characterized using a combination of quantum mechanical calculations and molecular dynamics simulations. This has allowed us to obtain information in atomic detail about each step of the reaction mechanism, that is, substrate entrance and achievement of the near-attack conformation, tr...

Pseudomonas putida PP3 grew on DL-2-monochloropropionate (2MCPA) with a release of chloride ions consistent with the dechlorination of both isomers. The organism grew on either D- or L-2MCPA. Dehalogenase activity in cell-free extracts showed that both D- and L-2MCPA were dehalogenated. Pseudomonas putida PP3 contains two dehalogenases, and studies with the separated enzymes revealed that the f...

Multifunctional nanoparticles (MNPs) that combine unique superparamagnetic properties and fluorescence emission are promising bimodal tracers for the noninvasive diagnosis of malignant cells both in vitro and in vivo. The selective recognition of specific cancer cells impacts diagnostic sensitivity, and it can be accomplished by the functionalization of MNPs with molecules that have a high affi...

Although reductive dehalogenation by anaerobic microorganisms offers great potential for the degradation of halocarbons, little is known about the biochemical mechanisms involved. It has previously been demonstrated that the dehalogenase activity involved in 3-chlorobenzoate dehalogenation by Desulfomonile tiedjei DCB-1 is present in the membrane fraction of the cell extracts. We report herein ...