Synopsis A short history of the laboratory diagnosis of pregnancy is followed by an evaulation of four immunological methods of pregnancy diagnosis. The immunological methods based upon haemagglutination-inhibition and slide latex agglutination, gave a.lOO% accuracy. It is concluded that these techniques are suitable for the diagnosis of pregnancy after the 35th day following the last menstrual...

an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...

The passive haemagglutination and bentonite flocculation tests were compared for detecting plant viruses, using antibody-sensitized tanned red cells or bentonite particles. Parsnip yellow fleck, raspberry ringspot, turnip yellow mosaic, narcissus mosaic, potato X and tobacco rattle viruses were detected by both tests in purified preparations and, where studied, in crude plant extracts. The high...

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...

The findings agree closely with those of Hobbs and Aw (1968). The isoamylase patterns of normal plasma and urine are similar, though the activity in urine is greater. Three bands are usually found in normal urine, the slower, and usually more distinct, band being the pancreatic component, and the faster those derived from salivary gland. The activities of these isoenzymes in plasma appear to be...

OBJECTIVES This study was designed to identify the optimal dose of an MF59-adjuvanted, monovalent, A/H1N1 influenza vaccine in healthy paediatric subjects. METHODS Subjects aged 3-8 years (n=194) and 9-17 years (n=160) were randomized to receive two primary doses of A/H1N1 vaccine containing either 3.75 μg antigen with half a standard dose of MF59 adjuvant, 7.5 μg antigen with a full dose of ...

INTRODUCTION Illness and death from influenza increase during pregnancy. In the United Kingdom pregnant women were targeted in a national programme for vaccination during the H1N1 2009-10 pandemic. METHODS In this study, pregnant women were recruited in labour from November 9, 2009 to March 10, 2010. Pandemic vaccination status was determined. Venous cord blood collected at delivery was evalu...

INTRODUCTION HIV infection is considered a risk factor for severe outcomes of influenza A(H1N1)v infection. However, data on immune response against influenza A(H1N1)v virus in HIV-infected patients are lacking. METHODOLOGY Data from seven HIV-positive and 14 HIV-negative patients infected with A(H1N1)v and from 23 HIV-positive and six HIV-negative asymptomatic controls were analyzed to evalu...

The evaluation of the contribution of neurological dengue in suspected central nervous system (CNS) viral infections is essential to better understand the impact of neurological dengue on morbidity and mortality in dengue endemic regions such as Jamaica. For this study 401 cases of suspected viral CNS infections were investigated for evidence of dengue infection. The frequency of neurological d...

OBJECTIVE To determine whether the novel avian influenza H7N9 virus can transmit from person to person and its efficiency. DESIGN Epidemiological investigations conducted after a family cluster of two patients with avian H7N9 in March 2013. SETTING Wuxi, Eastern China. PARTICIPANTS Two patients, their close contacts, and relevant environments. Samples from the patients and environments we...