نتایج جستجو برای: h2o2 priming
تعداد نتایج: 35451 فیلتر نتایج به سال:
Oxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress induced by hydrogen peroxide (H2O2) in ovine sperm motility after thawing and catalase (CAT) ability to preserve sperm motility. Semen was incubated at 37°C with 100 μM H2O2, 1 U catalase + 100 μM H...
OBJECTIVE We tested the hypotheses that low concentration of H2O2 attenuates the Ca2+ paradox (Ca2+ PD) injury, and that activation of protein kinase C (PKC) and/or ATP-sensitive potassium channel (KATP) are involved in the protective effects of H2O2. METHODS Langendorff-perfused rat hearts were subjected to the Ca2+ PD (10 min of Ca2+ depletion followed by 10 min of Ca2+ repletion). Function...
Hydrogen peroxide (H2O2) at moderate steady-state concentrations synergizes with TNF-α, leading to increased nuclear levels of NF-κB p65 subunit and to a cell-type specific up-regulation of a limited number of NF-κB-dependent genes. Here, we address how H2O2 achieves this molecular specificity. HeLa and MCF-7 cells were exposed to steady-state H2O2 and/or TNF-α and levels of c-Rel, p65, IκB-α, ...
Field experiments were conducted during 2017 and 2018 to evaluate the efficacy of herbicides NICOSULFURON and EPTC alone or in combination with seed priming on weed control and corn yield. NICOSULFURON (80 g a.i. ha‒1) was applied at four- to six-leaf stage of corn as post-emergence and EPTC (4920 g a.i. ha‒1) applied as pre-plant incorporation (PPI). Among the herbicide treatments, the lowest ...
The objective of this research was to evaluate the effect of salicylic acid and gibberellic acid on germination characteristics and changes of proline, protein and catalase activity of Brassica napus seedlings under Cu(SO4) stress. The experimental design was factorial with complete randomized design as a base design with 3 replications. The first factor was 4 levels of Cu(SO4) stress (0, 10, 2...
P-11: DHR123: A Suitable Fluorescent Mitochondrial Probe for Assessment of H2O2 in Human Spermatozoa
Background: The objective of present study was to assess potential of DHR123 (dihydrorhodamine 123) fluorescent probe for measurement of H2O2 produced by human spermatozoa and comparing the results with DCFH-DA (2', 7'-dichlorodihydrofluorescein diacetate). Materials and Methods: Fluorescent intensity and percentage R123 and DCF positive sperm measured by flow cytometry. The suitable ...
Seed germination, emergence and crop growth are often constrained by multiple environmental stress under field condition limiting productivity. priming, a age old inexpensive technique, consisting of soaking seeds in priming agent prior to sowing, has been applied different crops conditions with varying degrees success. To understand the significance this potentially transformative agronomic st...
In this study, expressions of toll-like receptors (TLRs) and apoptosis-related genes in piglets and mitochondrial respiration in intestinal porcine epithelial cells were investigated after hydrogen peroxide (H2O2) exposure. The in vivo results showed that H2O2 influenced intestinal expressions of TLRs and apoptosis related genes. H2O2 treatment (5% and 10%) downregulated uncoupling protein 2 (U...
In order to study of effect of on-farm seed priming and irrigation interval on growth indices of two corn cultivars, an experiment was conducted at the research farm of agriculture faculty of Bu-Ali Sina University, Hamedan-Iran in 2011. The experiment was a split plot factorial based on completely randomized block design with three replications. Experimental factors were including irrigation, ...
Hydrogen peroxide (H2O2) is produced by inflammatory and vascular cells and induces oxidative stress, which may contribute to vascular disease and endothelial cell dysfunction. In smooth muscle cells, H2O2 induces production of O2 by activating NADPH oxidase. However, the mechanisms whereby H2O2 induces oxidative stress in endothelial cells are not well understood, although O2 may play a role. ...
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