Attachment and ingestion of Mycobacterium avium subsp. paratuberculosis by two epithelial cell lines were enhanced by soluble fibronectin (FN). Peptide blocking of the FN attachment protein (FAP-P) inhibited the internalization of M. avium subsp. paratuberculosis. Disruption of FAP-P expression significantly reduced attachment and ingestion of M. avium subsp. paratuberculosis by T-24 and Caco-2...

The green alga Chlorogonium elongatum, a member of the Volvocales, possesses a crystalline cell wall composed of hydroxyproline-rich glycoprotein similar to the primary cell wall glycoproteins of higher plants. Electron microscopy and computer image processing have been used to determine the crystal structure of the Chlorogonium cell wall in three dimensions to a resolution of 2.0 nm. The struc...

gene that responds to DNA-damaging agents. J. Bacteriol. 172:3284-3289. McEvoy, J. L., K.K. Thum, and A.K. Chatterjee. 1987. Expression of the E. coli lexA+ gene in Erwinia carotovora subsp, carotovora and its effect on production of pectin lyase and carotovoricin. Fed. European Microbiol. Soc. Microbiol. Lett. 42:205–208. Mount, M.S., P.M. Berman, R.P. Mortlock, and J.P. Hubbard. 1979. Regulat...

Three actinomycetes having capability of 3 beta-hydroxylation of ML-236B were isolated from soil samples collected in Australia. Strain SANK 62781 was identified as Nocardia autotrophica. Strain SANK 62881 and strain SANK 62981 were identified as new subspecies of N. autotrophica for which the name N. autotrophica subsp. canberrica and N. autotrophica subsp. amethystina are proposed, respective...

The in vitro activities of new beta-lactam antibiotics was studied and compared with those of other known agents against 51 clinical isolates of Acinetobacter calcoaceticus subsp. anitratus and 23 isolates of A. calcoaceticus subsp. lwoffi. Of the new beta-lactam antibiotics, imipemide (N-formimidoyl thienamycin), ceftazidime, ceftizoxime, ceftriaxone, and piperacillin demonstrated good activit...

physiological effect of six levels of water stress (0, -0.1, -.03, -0.6, -0.9 and -1.2 mp) was studied on seed germination and plumule as well as radicule growth length in three species namely: agropyron elongatum, agropyron desertourm and secale montanum. polyethylene glycol (peg) was used to provide appropriate water potentials. a total of 100 seeds in four replications (petri dishes) were so...

Campylobacter fetus subsp. fetus and C. fetus subsp. venerealis are currently differentiated by tolerance to glycine and by their epidemiology. Analysis of C. fetus DNA by pulsed-field gel electrophoresis, after digestion with the restriction endonucleases SmaI and SalI, was used to differentiate between the subspecies. All strains presently identified as C. fetus subsp. fetus had a genomic siz...

A new detection test for the mycoplasmas causing contagious agalactia, Mycoplasma agalactiae, M. capricolum subsp. capricolum and M. mycoides subsp. mycoides L. C., was developed. It was based on two polymerase chain reaction assays: the Ma-PCR for the detection of M. agalactiae and the MYC-PCR for the 'mycoides cluster' thus including M. capricolum subsp. capricolum and M. mycoides subsp. myco...

AIMS To investigate the growth and release of Lactococcus lactis subsp. lactis in gel beads and to affect rates of cell release by changing the growth conditions. METHODS AND RESULTS The rate of release and the distribution of immobilized L. lactis subsp. lactis in alginate beads were studied in continuous fermentations for 48 h. A change in operating pH from 6.5 to 9.25 initially reduced the...