Abstract The effect of four diets on the performance Dorper lambs that were artificially mix-infected with 1000 infective larvae (L3) Haemonchus contortus and L3 Trichostrongylus colubriformis every three days for 12 weeks was evaluated. For each diet, one infected group (n = 7) control 4) set up. contained following proportions hay (H) concentrate (C): Diet 1 - 100%H; 2 75%H:25%C; 3 50%H:50%C;...

background: to use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. methods: peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of hae­monchus contortus were determined using sds-page and western blotting. five lambs were infected with 10,000 third stage larv...

Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a s...

Surveys to determine the prevalence and degree of resistance of Haemonchus spp. of sheep and goats to the available anthelmintics in South Africa indicate that small ruminant production is entering a crisis situation. Three surveys employing the faecal egg count reduction (FECR) test to determine resistance were conducted in some of the main sheep-producing areas in the summer rainfall region o...

A reduction in live weight occurred in the pen-fed sheep until they became accustomed to the lucerne pellets. In the pen-fed sheep, but not in the paddock sheep, the effects of a sub-clinical infection of H. contortus were observed to cause a significant but transient decrease in plasma insulin-like growth factor 1 (IGF-1). The reduced plasma IGF-1 concentrations suggest that a further decline ...

Ten female lambs of 7-month-old were divided into two equal groups and raised under a helminth-free conditions. Animals in group 1 were immunized two times by whole gut homogenate (WGH) of Haemonchus contortus emulsified in Freund’s adjuvant. In group 2 (control), animals were injected by phosphate buffered saline emulsified in the same adjuvant. Animals were challenged by 10000 third-stage lar...

Risk can be defined as the possibility of loss or injury coupled to the probability of such loss or injury (Singh 2000), and the main components of risk analysis are risk assessment, risk management and risk communication (Thrusfield 2001). Quantitative risk assessment is defined as a mathematical model containing inputs and outputs which are expressed numerically (Murray 2004). The work in the...

Haemonchus contortus and Trichostrongylus colubriformis are among the most important parasitic nematodes of small ruminants. Caenorhabditis elegans, a free-living nematode, is used as a model for evaluating anthelmintic activity of a variety of test substances. Extracts of several medicinal plants are useful in vitro and in vivo against nematode development. Extracts of Curtisia dentata, a Sout...