H5N1 avian influenza viruses are a major pandemic concern. In contrast to the highly virulent phenotype of H5N1 in humans and many animal models, guinea pigs do not typically display signs of severe disease in response to H5N1 virus infection. Here, proteomic and transcriptional profiling were applied to identify host factors that account for the observed attenuation of A/Tiger/Harbin/01/2002 (...

The Armstrong CA 1371 (ARM) and WE strains of lymphocytic choriomeningitis virus (LCMV) differ in the ability to produce disease in adult guinea pigs. Infection with the ARM strain is not lethal, even at high virus doses (greater than 10,000 PFU), whereas the WE strain causes 100% mortality even at low doses (less than 10 PFU). To determine the genetic basis of this virulence, intertypic reasso...

Cholesterol absorption was studied in groups of guinea pigs fed diets containing 0, 0.1%, or 1% cholesterol. A similar proportion of tracer cholesterol was absorbed regardless of the cholesterol content of the diet. Furthermore, the proportion of tracer cholesterol absorbed by individual animals did not change when the cholesterol-free diet was changed to one containing 1% cholesterol. Choleste...

OBJECTIVE To develop an animal model of intraocular tuberculosis (TB) with features of pulmonary TB and extrapulmonary dissemination to the eye. METHODS Hartley strain guinea pigs were infected via an aerosol route with virulent Mycobacterium tuberculosis. One group of guinea pigs was infected with a relatively low bacterial inoculum and received no treatment. A second group of guinea pigs re...

Guinea pigs infected with low-passage Junin virus of human origin showed viral strain dependent differences in mortality, LD50, time to death, and in viral spread and distribution. Different Junin strains appeared to cause at least two broad patterns of Argentine hemorrhagic fever in guinea pigs. A number of strains of Junin virus caused a viscerotropic type of illness in which virus replicated...

1. In rabbits, guinea pigs, and mice pneumococcus autolysate antitoxin has no effect against generalized infection by the pneumococcus. 2. In a large proportion of cases of pneumonia and sepsis caused by the intratracheal inoculation of large amounts of living pneumococci in normal or sensitized guinea pigs, autolysate antitoxin saves the animal. 3. The autolysate antitoxin is protective in mos...

PURPOSE To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of ...

The protective effects of polyclonal antisera produced by injecting guinea pigs with protective antigen (PA), the chemical anthrax vaccine AVA, or Sterne spore vaccine, as well as those of toxin-neutralizing monoclonal antibodies (MAbs) produced against PA, lethal factor, and edema factor, were examined in animals infected with Bacillus anthracis spores. Only the anti-PA polyclonal serum signif...

We studied the type specificity of the antisera which were produced in guinea pigs by immunization with heat-disrupted virions of human enteroviruses (echovirus types 7 and 11 and coxsackievirus type B5). When guinea pigs were immunized with heated virions (H particles), the antibodies produced reacted type specifically by complement fixation with the homologous H particles. By contrast, when g...