citric acid and tetracycline hcl are the most common clinical root demineralization agents used in periodontal practice. in this comparative in vitro study the effects of nd: yag laser radiation on root surface was compared to that of citric acid and tetracycline hcl using sem. a total of twenty-one freshly extracted periodontally diseased single rooted premolar teeth were root planned in vitro...

Abstract Recent studies have revealed the prevalence and biological significance of guanidine metabolism in nature. However, metabolic pathways used by microbes to degrade or mitigate its toxicity not been widely studied. Here, via comparative proteomics subsequent experimental validation, we demonstrate that Sll1077, previously annotated as an agmatinase enzyme model cyanobacterium Synechocyst...

Purification of lectin from the seeds of Crotalaria medicaginea Lamk by affinity chromatography on asialofetuin-linked amino activated silica, yielded a single band on non-denatured PAGE at pH 4.5 and 8.3 and, a single peak on HPLC size exclusion and cation exchange columns. The molecular mass of the native C. medicaginea lectin was determined to be 125 kDa by gel filtration. In SDS-PAGE, the l...

protein engineering but also for practical purposes such as potential cleaning agents, food additives, and dehairing agents. Recently, we isolated many alkalophilic actinomycetes as in our previous paper.X) One of the interesting alkalophilic actinomycetes, Nocardiopsis dassonvillei subsp. prasina OPC-210, produced two types of alkaline serine proteases (NDP-I and NDP-II). We have already repor...

Affinity chromatography has been applied to the problem of specifically removing luteinizing hormone from sera used to supplement tissue culture media. Horse or fetal calf sera, passed through a column of an anti-luteinizing hormone immunoglobulin fraction linked covalently to agarose, were specifically depleted in their content of luteinizing hormone as seen by radioimmunoassay and by their ef...

Salmonella PQrQtyphi B SL 877 was shown to be a suitable source of flagellar protein whose amino acid sequence was determined by the H-l structural gene of Salmonella fyphimurium. Isolated flagellin gave one band at a variety of pH levels in acrylamide gel electrophoresis in the presence of disaggregating agents and contained a small amount of carbohydrate. The amino acid content of this flagel...

Several ras genes have been expressed at high levels in Escherichia coli and the resultant ras proteins were shown to be functional with respect to their well-known specific, high-affinity, GDP/GTP binding. We were able to detect a weak GTPase activity associated with the purified proteins. The normal cellular ras protein (p21N) exhibits approximately equal to 10 times higher GTPase activity th...

METHODS Spectral and Anaerobic Measurements. All absorbance spectra and anaerobic titrations employed the thermostatted Milton Roy Spectronic 3000 diode array spectrophotometer. The oxidoreductase and DTNB assays were performed on a thermostatted Applied Photophysics DX.17MV stopped-flow spectrofluorometer. The molar extinction coefficients of the proteinbound FAD at 448 or 449 nm were determin...

The cell envelope of Mycobacterium tuberculosis (Mtb) is complex and diverse; composed of proteins intermingled in a matrix of peptidoglycan, mycolic acids, lipids, and carbohydrates. Proteomic studies of the Mtb cell wall have been limited; nonetheless, the characterization of resident and secreted proteins associated with the cell wall are critical to understanding bacterial survival and immu...

Decorin is a widely distributed member of the extracellular matrix small leucine-rich repeat glycoprotein/proteoglycan family. For investigation of its physical properties, decorin from two sources (young steer skin and a recombinant adenovirus) was used. The first sample was extracted into 7 m urea and purified, while the second was isolated from medium conditioned by 293A cells infected with ...