Trimeresurus gracilis is an endemic alpine pitviper in Taiwan with controversial phylogeny, and its venom proteome remains unknown. In this study, we conducted a proteomic analysis of T. using high-performance liquid chromatography-tandem mass spectrometry identified 155 toxin proteoforms that belong to 13 viperid families. By searching the sequences trypsin-digested peptides separated HPLC fra...

Secreted phospholipases A2 (sPLA2s) form a widespread group of structurally-related enzymes that catalyse the hydrolysis sn-2 ester bond glycerophospholipids to produce free fatty acids and lysophospholipids. In humans, nine catalytically active two inactive sPLA2 proteins have been identified. These play diverse biological roles, including host defence against bacteria, parasites viruses. Seve...