Abbreviations used: HIV, human immunodeficiency virus ; SIV, simian immunodeficiency virus; Env, envelope glycoprotein, VSV-G, vesicular stomatitis virus glycoprotein; GPCR, G-protein coupled receptor; CKR, chemokine receptor, PTX, Bordetella pertussis toxin; SH3, src homology-3; PACS-1, phosphofurin acidic cluster sorting protein-1; MHC, major histocompatibility class; GFP, green fluorescent p...

IgG antibody-transporter conjugates enable intracellular uptake of biologically active IgG antibodies that inhibit viral mediated syncytia formation in respiratory syncytial virus green fluorescent protein (RSV-GFP) infected human epithelial cells (HEp-2).

The green fluorescent protein (GFP) is currently being used for diverse cellular biology approaches, mainly as a protein tag or to monitor gene expression. Recently it has been shown that GFP can also be used to monitor the activation of second messenger pathways by the use of fluorescence resonance energy transfer (FRET) between two different GFP mutants fused to a Ca2+sensor. We show here tha...

Green fluorescent protein (GFP) and its relatives (GFP protein family) have been isolated from marine organisms such as jellyfish and corals that belong to the phylum Cnidaria (stinging aquatic invertebrates). They are intrinsically fluorescent proteins. In search of new members of the family of green fluorescent protein family, we identified a non-fluorescent chromoprotein from the Cnidopus ja...

The green fluorescent protein (GFP) of Aequorea victoria has been developed here as a reporter for gene expression and protein localization in Candida albicans. When wild-type (wt) GFP was expressed in C. albicans, it was not possible to detect fluorescence or a translation product for the wt protein. Since this was probably due in part to the presence of the non-canonical CTG serine codon in t...

Green fluorescent proteins (GFPs) and variants thereof are widely used to study protein localization and dynamics. We engineered a specific binder for fluorescent proteins based on a 13-kDa GFP binding fragment derived from a llama single chain antibody. This GFP-binding protein (GBP) can easily be produced in bacteria and coupled to a monovalent matrix. The GBP allows a fast and efficient (one...

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but ...

We have utilized multicolored fluorescent proteins to develop three imaging models of tumor angiogenesis. In one model, the nonluminous induced capillaries are clearly visible by contrast against the very bright tumor green fluorescent protein (GFP) fluorescence examined either intravitally or by whole-body imaging in real time. Intravital images of an orthotopic model of human pancreatic tumor...

eosinophil autofluorescence. J. Leukoc. Biol. 51:172-175. 12.Nokubo, M., M. Ohta, K. Kitani and I. Z.Nagy. Identification of protein-bound riboflavin in rat hepatocyte plasma membrane as a source of autofluorescence. Biochim. Biophys. Acta 981:303-308. 13.Olson, K.R., J.R. McIntosh and J.B. Olmsted. 1995. Analysis of MAP4 function in living cells using green fluorescent protein (GFP) chimeras. ...

Penicillium griseoroseum, a deuteromycete fungus producer of pectinolytic enzymes, was transformed with a gene encoding for green fluorescent protein (GFP). The selection of transformants was based on the homologous nitrate reductase gene (niaD). Protoplasts of a P. griseoroseum Nia mutant (PG63) were co-transformed with the plasmids pNPG1 and pAN52-1-GFP. The plasmid pNPG-1 carries the homolog...