BACKGROUND To test whether poor quality day-3 embryos can undergo successful blastulation and implantation. METHODS A prospective cohort study was conducted. Whether or not a good quality embryo was transferred on day-3, poor quality (rejected) embryos were further cultured and followed. The clinical outcome of each embryo was assessed. RESULTS A total of 694 rejected embryos (from 205 pati...

Abstract Study question Can blastocyst shrinkage-pattern during vitrification be predictive of post-warming survival and reproductive potential? Summary answer Blastocysts assuming a specific are liable to lower morphology and, potentially, degeneration. What is known already Blastocyst highly standardized technique. However, there still possible factors than can compromise the outcome techniqu...

Objective One of the most controversial issues related to human embryo is to determine the moment when the embryo is considered as a human being and acquires a moral status. Although personhood and moral status are frequently touched upon in medical ethics, they are considered interdisciplinary as concepts shaping the debate in Medical Law (Fiqh) since their consequences are influential in the ...

one studies the $hx$-hypergroups, corresponding to the chinese hypergroups associated with the direct products of some ${bf z}/n {bf z},$ calculating their fuzzy grades.

in this study we have described our varicocele cases in terms of associated symptoms/signs. in addition, we have tested the correlation between semen parameters and varicocele grades and compared the relative frequencies of associated symptoms/signs in different varicocele grades. 1 19 referred cases of varicocele were categorized based on clinical criteria into 3 grades by one examiner. the re...

Abstract Study question Can iDAScore predict ongoing clinical pregnancy (CP) with a sensitivity and specificity equivalent to that associated manual morphology assessment grading? Summary answer The fully automated was able CP performance grading in this retrospective cohort study. What is known already an artificial intelligence (AI) based algorithm developed by applying machine learning morph...

BACKGROUND The purpose of this study was to evaluate the respective influences of blastomere survival and resumption of mitosis on the outcome of frozen-thawed embryos. METHODS A retrospective analysis was performed in our centre on 363 thawing cycles, involving 4-cell day 2 grade 1 embryos with <10% fragmentation. RESULTS A higher implantation rate per transferred embryo was observed when ...

We investigated gene expression associated with trophectoderm epithelial intercellular junction formation in single human embryos at different stages of cleavage using RT-PCR methods based upon magnetic bead separation of polyA+ RNA. Trophectoderm tight junction (TJ) and desmosome biogenesis contribute to intercellular sealing and tissue integrity, critical for vectorial transport and blastocoe...

The identification of transcription factor (TF) binding sites in the genome is critical to understanding gene regulatory networks (GRNs). While ChIP-seq is commonly used to identify TF targets, it requires specific ChIP-grade antibodies and high cell numbers, often limiting its applicability. DNA adenine methyltransferase identification (DamID), developed and widely used in Drosophila, is a dis...

Objective: To investigate the effect of improved air quality on IVF and subsequent embryo development. Design: Retrospective cohort study. Setting: Hospital-based IVF facility composed of an anteroom, a cleanroom, and an adjacent operating room. Patient(s): Two-hundred seventy-five couples requesting IVF between 1993 and 1997. Intervention(s): None. Main Outcome Measure(s): Particle counts (siz...