Dentinogenesis is a necessary prerequisite for dental tissue engineering. One of the steps for dentinogenesis is to obtain large quantities of highly purified odontoblasts. Therefore, we have undertaken an experiment applying different concentrations of β-glycerophosphate (β-GP) to induce the differentiation of dental pulp stem cells (DPSCs) in a long-term 28-day culture. In the meanwhile, we h...

Lipophilic and hydrophilic D-alanyl-lipoteichoic acids are elongated in Lactobacillus casei by the transfer of sn-glycerol 1-phosphate units from phosphatidylglycerol to the poly(glycerophosphate) moiety of the polymer. These sn-glycerol 1-phosphate units are added to the end of the poly(glycerophosphate) which is distal to the glycolipid anchor; 1,2-diglyceride results from this addition. The ...

The activity of acyl-CoA: dihydroxyacetone phosphate acyltransferase in rat liver was studied by measuring the formation of labeled lipid from dihydroxyacetone [W]phosphate and either palmitoyl-CoA or a mixture of palmitate, CoA, and ATP. Bovine serum albumin stimulated the activity of the enzyme several-fold. In the presence of albumin the acyltransferase activity in mitochondria was much high...

The main aim of this study was to investigate whether enzyme levels of the malate-aspartate and alpha-glycerophosphate shuttles and of cytochrome b5 reductase in human skeletal muscle are affected by strength training. Muscle biopsy samples from the deltoid muscle of the nondominant arm in untrained (n = 12) and strength-trained (n = 12) subjects were compared. The strength-trained muscles were...

A capsulated strain of derobacter aerogenes 1033 (2) has been found to metabolize glycerol via two separate pathways. The first pathway was mediated by a diphosphopyridine nucleotidelinked glycerol dehydrogenase and a specific dihydroxyacetone kinase, whereas the second pathway involved a specific glycerol kinase and a DPN-independent L-a-glycerophosphate dehydrogenase (3-5). Although all the a...

Polar ether lipids of Thermoplasma acidophilum HO-62 were purified by high-performance liquid chromatography with an evaporative light-scattering detector. Structures of purified lipids were investigated by capillary gas chromatography, mass spectrometry, and nuclear magnetic resonance. Three types of ether lipids were found: phospholipids, glycolipids, and phosphoglycolipids. The two phospholi...