Introduction: Skeletal staining is an important part of anatomical studies which can evaluate macroscopic disorders in bones and cartilages development. The aim of the present article is to illustrate two distinct protocols by all details in order to stain skeletal system of the lab animals in all ages of their lives so it becomes easy for future studies in this field. Methods: In this pa...

The amino acid-producing organism Corynebacterium glutamicum cannot utilize glycerol, a stoichiometric by-product of biodiesel production. By heterologous expression of Escherichia coli glycerol utilization genes, C. glutamicum was engineered to grow on glycerol. While expression of the E. coli genes for glycerol kinase (glpK) and glycerol 3-phosphate dehydrogenase (glpD) was sufficient for gro...

1. The possible role of glycerol as a precursor in neonatal gluconeogenesis in the rat was investigated by recording the activities of glycerol kinase and l-glycerol 3-phosphate dehydrogenase in the liver, kidney and other tissues around birth and during the neonatal period. 2. Blood glycerol concentrations in the neonatal rat are high. 3. There is a marked increase after birth in the ability o...

1. Glycerol kinase (EC 2.7.1.30) is shown to catalyse a non-equilibrium reaction in rat liver; and, as it is the first enzyme in the pathway metabolizing glycerol, its properties may be pertinent to the metabolic regulation of glycerol uptake and utilization by this tissue. 2. The properties of hepatic glycerol kinase were studied by using a radiochemical technique to measure the enzyme activit...

Jensen, Michael D. Regional glycerol and free fatty acid metabolism before and after meal ingestion. Am. J. Physiol. 276 (Endocrinol. Metab. 39): E863–E869, 1999.—We measured splanchnic and leg glycerol [and free fatty acid (FFA)] uptake and release in 11 healthy volunteers before and after meal ingestion to assess whether regional FFA-to-glycerol release ratios mirror systemic release ratios. ...

STEELE, R., B. WINKLER, AND N. ALTSZULER. Inhibition by infused glycerol of gluconeogenesis from other precursors. Am, J. Physiol. 22 l(3) : 883-888. 1971 .-Plasma glycerol contributes 20% of the carbon of glucose released to the blood by the liver in the 6to 7-dayfasted dog (shown by use of glycerol-UJ4C). This increases to 63y0 when bulk glycerol infusion increases total glycerol uptake from ...

BERGMAN, E. N. Glycerol furnouer in the nonpregnant and k&tic pregnant sheep. Am. J. Physiol. 215(4): 865-873. 1968.Labeled glycerol was infused intravenously into three groups of sheep: fed, fasted, and twin-pregnant hypoglycemic and ketotic. The turnover time of the free glycerol pool was rapid in all animals (15-l 8 min). Mean glycerol turnover rates were 0.25 mmole/hr per kg3i4 in fed anima...

mono-specific antisera against vibrio cholera ogawa nih-43 and vibrio cholera inaba 35-a3 were prepared from rabbit hyperimmune sera by absorbing against a heterologous strain. using ammonium sulphate precipitation procedure, gamma globulins were purified and concentrated. to visualize antigen-antibody reaction, gamma globulins were conjugated to staphylococcus aureus cowan-l (nctc: 8325) in th...

Although glycerol is the primary carbon source available to halophilic heterotrophic communities, little is known regarding haloarchaeal glycerol metabolism. In this study, a gene encoding a glycerol kinase homolog (glpK; HVO_1541) was deleted from the genome of the haloarchaeon Haloferax volcanii by a markerless knockout strategy. The glpK mutant, KS4, readily grew on yeast extract-peptone com...

background and objectives: preparation and evaluation of nanocomposite films have become prevalent in recent years. depending on the purpose of using the film, and considering the effects and interaction effects of nanomaterial and other modifiers on the film properties, the amounts of nanomaterials and modifiers should be optimized. the effects of montmorillonite (mmt), glycerol (gl), and thei...