Reaction of F-actin and the F-actin-tropomyosin complex with 20 mM glutaraldehyde for 19-22 h at 0 degrees C and 25 degrees C results in extensively cross-linked filaments, as judged by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. Electron micrographs show shorter, more irregular filaments for glutaraldehyde-treated F-actin in the absence of tropomyosin as compared to the pr...

As part of an overall evaluation of possible substitutes for the pulpotomy agent formocresol, this study was initiated to compare the antigenicity of the reaction products of protein with formaldehyde, glutaraldehyde, or dimethylsuberimidate (DMS). Rabbits were injected with rabbit serum albumin (RSA) which had been treated with one of the following solutions: phosphate-buffered saline, 2% glut...

BACKGROUND AND AIMS The laryngoscope is a potential source of cross-infection as it involves contact with the mucous membrane, saliva and occasionally blood. This study compared efficacy and cost-effectiveness of two Centre for Disease Control approved agents for disinfection of laryngoscope blades. METHODS One hundred and sixty patients requiring laryngoscopy and intubation for general anaes...

BACKGROUND AND AIMS The most common method for alginate impression disinfection is spraying it with disinfecting agents, but some studies have shown that these impressions can be immersed, too. The aim of this study was to evaluate the dimensional stability of alginate impressions following disinfecting by spray and immersion methods. MATERIALS AND METHODS Four common disinfecting agents (Sod...

In this report we examined the antigen-presenting cell (APC) requirements for activation of T-cell hybridomas specific for the protein antigen PPD (purified protein derivative of tuberculin). During the course of these studies we observed that glutaraldehyde fixation of Ia-positive A20.2JAD (A20) and P388D1 stimulator cells had different effects on T-cell activation. A20 cells fixed with glutar...

The effects of time, concentration, and pH on glutaraldehyde ( GA) fixation were studied in vitro using 2 model systems: coIlagen-BSA gels as simulations of cell cytoplasm and enzyme activity in treated pulp tissue. The former system demonstrated that GA is most effective when buffered, and that its penetration is self-limiting. In general, concentration proved more a factor in the depth of pen...

It has been shown that the addition of a beta-adrenergic catecholamine to a trout red blood cell suspension induces a 60-100-fold increase of sodium permeability resulting from the activation of a cAMP-dependent Na+/H+ antiport. Subsequent addition of propranolol almost instantaneously reduces the intracellular cAMP concentration, and thus the Na permeability, to their basal values (Mahé et al....