The distribution and mobility of anionic sites on the surfaces of baby hamster kidney cells were studied by utilizing the multivalent ligand, polycationic ferritin, as a visual probe. Our observations revealed that anionic sites are distributed over the entire cell surface, with the highest density of sites being located on cell surface microextensions. Following the initial binding of polyca...

Supporting structures derived from biological tissue have been used in numerous tissue-engineering applications. This study focuses on the immune response of human leukocytes toward decellularized or glutaraldehyde (GA) cross-linked vascular tissue in vitro. Porcine and human pulmonary roots were sterilized with antibiotics, decellularized or cross-linked with GA. Proteins of the vascular tissu...

The fine structure of young root hairs of radish was studied, with special attention to cytoplasm-wall relationships. Hairs up to 130 micro in length were examined after fixation of root tips in glutaraldehyde followed by osmium tetroxide. Microtubules occur axially aligned in the cytoplasm just beneath the plasmalemma, and extend from the base of the hair to within 2 to 3 micro of the tip. Pos...

MIC-tests in replicate were performed on 286 Salmonella isolates (269 from Danish poultry, including 256 from broiler houses, and 17 from England, mainly from poultry) against five disinfectants used in the poultry industry (glutaraldehyde/benzalkonium chloride compound, formaldehyde, oxidising compound, tar oil phenol, iodophor). Generally, the small variations in MIC could not be associated w...

Scanning electron microscopic (SEM) studies of tile cephalic region of members of the fainily Heteroderidae have failed to reFort inner labial sensilla of second-stage larvae, ahhough the labial sensilla of males have been observed (1,4,6,7,9,11,12,13). T h e existence and location of the labial sensilla in tile families Heteroderidae and Meloidogynidae have been discussed among hematologists c...

Histones have been fixed within the chromatin complex using either formaldehyde or glutaraldehyde. Evidence is presented which argues that in short time periods formaldehyde fixation leads to the formation of reversible covalent bonds between histone and DNA. On the other hand, fixation of chromatin with glutaraldehyde leads initially to the formation of polymers of F1 histone, and at a later s...

A vaccination protocol against murine Babesia microti infection, using glucan, a beta-1,3-glucopyranose derivative of yeast cell walls, and glutaraldehyde-fixed infected erythrocytes was evaluated. BALB/c mice were immunized intravenously four times at 2-day intervals with 2 X 10(8) fixed infected erythrocytes with and without glucan (0.45 mg). The mice were challenged 2 weeks after the last im...

This paper demonstrates the translational movement along the plane of the human erythrocyte ghost of the membrane particles exposed by freeze-fracture. The membrane particles can be aggregated by incubation of the ghosts in media with a pH in the vicinity of 5 5 or 3 5. The particles are disaggregated in neutral and alkaline media (pH 9 5) and also at pH 4.5 Aggregation of the particles at pH 5...

Cleaning and Disinfection • Little is known about virus survival outside the host; however, HEV is thought to be somewhat stable in the environment since feces are an important source of infection. • Heating pork products to an internal temperature of 71°C (160°F) for 20 minutes is required to completely inactivate HEV. • HEV is potentially susceptible to disinfection with acids like acetic aci...

The kinetic properties of a glutaraldehyde-modified phosphorylase b have been examined to understand further the mechanism of allosteric transition of this enzyme. The sigmoidal response to AMP, which is observed with native glycogen phosphorylase b, cannot be demonstrated with the modified enzyme. This enzyme derivative also exhibits no homotropic cooperativity of glucose 1 -phosphate under co...