1. A reinvestigation of the methods for the purification of ox-spleen P-glucuronidase using the variable solvent solubility test has revealed the presence oftwo fractions having different pH optima. The two enzymes have been separated and purified by fractional precipitation with ammonium sulphate and shown to have the foll6wing pH optima: with 1-menthylglucuronide 4-5 and 5 0; phenylglucuronid...

Clostridium perfringens was isolated from bile in 13 cases of 150 patients examined. The serotypes of C perfringens strains isolated from bile and faeces were investigated using antisera to Hobbs' type 1-17. Two or more serological types were often found in a single specimen, but in the same patient the serotypes of C perfringens strains isolated from the bile were identical with those from the...

1. d-Glucuronolactone was converted into d-glucaro-(1-->4)-lactone, a beta-glucuronidase inhibitor, probably via the intermediate d-glucaro-(1-->4)-(6-->3)-dilactone, by a dehydrogenase in human-liver extracts. 2. Similar experiments with mouse-liver extracts appeared to yield only d-glucaric acid or a non-inhibitory lactone. 3. A strong beta-glucuronidase inhibitor was present in mouse liver a...