Glucosidase II, an asparagine-linked oligosaccharide processing enzyme, is a resident glycoprotein of the endoplasmic reticulum. In kidney tubular cells, in contrast to previous findings on hepatocytes, we found by light and electron microscopy immunoreactivity for glucosidase II predominantly in post-Golgi apparatus structures. The majority of immunolabel was in endocytotic structures beneath ...

The extracellular β-glucosidase from microorganisms is generally produced in low levels. Therefore, in this study, a β-glucosidase hyperproducing mutant was developed by multiple exposures of ethyl methyl sulfonate (EMS) and ultraviolet (UV) radiation (both individually and jointly) to Bacillus subtilis strain (PS). The developed mutants were screened, selected and characterized. The mutant, PS...

Mycobacterium fortuitum and Mycobacterium chelonei are distinguished unambiguously by the combined use of five test characters: nitrate reductase, beta-glucosidase, acid production from fructose, penicillinase, and trehalase. Typically, M. fortuitum was nitrate reductase positive, beta-glucosidase positive; M. chelonei was nitrate reductase negative, beta-glucosidase negative, penicillinase pos...

Soil quality (SQ) assessment is a proactive process for evaluating soil and crop management effects on biological, chemical, and physical indicators of soil health. Our objectives were to evaluate several SQ indicators within five Agricultural Research Service (ARS) experimental watersheds (WS) and determine if those indicators were affected by manure, tillage, or crop rotation histories. Ten s...

in an attempt to develop alternative medicine for the treatment of diabetes and related complications, the antidiabetic potential of the fruits of c rataegus pinnatifida was evaluated. the antidiabetic potential of the methanol (meoh) extract as well as different solvent soluble fractions of the fruits of c . pinnatifida was evaluated via α-glucosidase, protein tyrosine phosphatase 1b (ptp1b), ...

Diabetes mellitus is a multifactorial metabolic disease characterized by post-prandial hyperglycemia (PPHG). α-amylase and α-glucosidase inhibitors aim to explore novel therapeutic agents. Herein we report the promises of Dioscorea bulbifera and its bioactive principle, diosgenin as novel α-amylase and α-glucosidase inhibitor. Among petroleum ether, ethyl acetate, methanol and 70% ethanol (v/v)...

The ability of probiotic strain Lactobacillus plantarum LS/07 to modify the activity of intestinal bacterial enzymes - β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in prevention of chronic diseases - cancer, atherosclerosis and dysbiosis was investigated. The male Sprague-Dawley rats were randomly divided into 12 experimental groups: controls groups - C (contro...

Objective: The objective of this study was to evaluate αamylase inhibitory and α – glucosidase inhibitory activity of the ethanolic extract of Dioscorea villosa tubers in vitro. Materials and Methods: Different concentrations of the plant extract (1.5, 3, 7, 15.30, 60, 125, 250, 500 and 1000 μg/ml) were prepared in ethanol and subjected to α amylase inhibitory and α Glucosidase inhibitory assay...

Alpha glucosidase inhibitors have been shown to be associated with pneumatosis intestinalis (PI) in recent reports. We herein report the case of a 73-year old man who received treatment with an alpha glucosidase inhibitor (acarbose) and presented with acute abdomen. A computed tomography scan demonstrated PI in addition to intrahepatic portal air and pneumoperitoneum. During exploratory laparot...

OBJECTIVE To evaluate the anti-hyperglycemic potential of sinigrin using in vitro, in silico and in vivo streptozotocin (STZ) induced hyperglycemic zebrafish model. METHODS The in vitro enzyme inhibition assay was carried out to determine the IC50 value against α-glucosidase and α-amylase, in silico molecular docking was performed against both enzymes with PyRx tool and simulations were perfo...