Three antipeptide antibodies were prepared by immunizing rabbits with synthesized short peptides corresponding to residues 215-226, 466-479, and 478-492 predicted from the cDNA of both the human hepatoma HepG2 and rat brain glucose transporters. All three antibodies were found to precipitate quantitatively the [3H]cytochalasin B photoaffinity-labeled human erythrocyte glucose transporter. Each ...

OBJECTIVE To identify possible mechanisms linking obesity in pregnancy to increased fetal adiposity and growth, a unique mouse model of maternal obesity associated with fetal overgrowth was developed, and the hypothesis that maternal obesity causes up-regulation of placental nutrient transporter expression and activity was tested. METHODS C57BL/6J female mice were fed a control (C) or a high-...

We have previously shown that Prochlorococcus sp. SS120 strain takes up glucose by using a multiphasic transporter encoded by the Pro1404 gene. Here, we studied the glucose uptake kinetics in multiple Prochlorococcus strains from different ecotypes, observing diverse values for the Ks constants (15-126.60 nM) and the uptake rates (0.48-6.36 pmol min-1 mg prot-1). Multiphasic kinetics was observ...

BY C A L L U M LIVINGSTONE, F I O N A J . T H O M S O N , M A R G A R E T I . A R B U C K L E , I A N W. CAMPBELL, THOMAS J . JESS , SUSAN K A N E , COLIN MOYES, LISA M. P O R T E R , J A C Q U E L I N E E . R I C E , M I C H A E L J . S E A T T E R A N D GWYN W. G O U L D Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G...

In diabetes, both activation of renin-angiotensin system and glomerular hyperfiltration are seen even before the development of diabetic nephropathy. Glomerular hyperfiltration depends on glomerular hypertension sue to pathophysiological afferent arteriolar dilation. Tubular hypothesis has been proposed more than a decade ago to explain the above in type1 diabetes. Recently, clinical evidences ...

SGLT2 inhibitors are new antihyperglycaemic agents whose ability to lower glucose is directly proportional to GFR. Therefore, in chronic kidney disease (CKD) the blood glucose lowering effect is reduced. Unlike many current therapies, the mechanism of action of SGLT2 inhibitors is independent of insulin action or beta-cell function. In addition, the mechanism of action of SGLT2 inhibitors is co...

The antipsychotic drug olanzapine is widely prescribed to treat schizophrenia and other psychotic disorders. However, it often causes unwanted side effects, including diabetes, due to disruption of insulin-dependant glucose metabolism through a mechanism yet to be elucidated. To determine if olanzapine can affect the first step in glucose metabolism - glucose transport inside cells - we investi...

Bai, Liqun, Xiaohong Zhang, and Fayez K. Ghishan. Characterization of vesicular glutamate transporter in pancreatic and -cells and its regulation by glucose. Am J Physiol Gastrointest Liver Physiol 284: G808–G814, 2003. First published November 20, 2002; 10.1152/ajpgi.00333. 2002.—Glutamate has been suggested to play an important role in the release of insulin and glucagon from pancreatic cells...

The article analyzes the main clinical and theoretical data on features of treatment atrial fibrillation (AF) in combination with diabetes mellitus (DM). Differentiated approaches to correction these cardiac arrhythmias this category patients are considered, among which methods first choice hypoglycemic therapy catheter ablation. Currently, addition metformin, is basis for most type 2 diabetes,...