Podocalyxin represents a Wilms' tumor suppressor protein (WT1)-regulated differentiation marker for glomerular epithelium. We provide evidence concerning mechanisms involved in the regulation of podocalyxin expression following long-term exposure to increased (25 mM) glucose levels. Prolonged culture of conditionally immortalized human podocytes in 25 mM glucose induced suppression of podocalyx...

BACKGROUND Post-translational modification regulates promoter-binding by Adr1, a Zn-finger transcriptional activator of glucose-regulated genes. Support for this model includes the activation of an Adr1-dependent gene in the absence of Adr1 protein synthesis, and a requirement for the kinase Snf1 for Adr1 DNA-binding. A fusion protein with the Adr1 DNA-binding domain and a heterologous activati...

Dark-cutting beef is a condition in which fails to have characteristic bright-red color when the cut surface exposed oxygen. However, mechanistic basis for this occurrence not clear. Protein expression profiles were compared between dark-cutting and normal-pH using LC-MS/MS-based proteomics. Mass spectrometry analysis identified 1162 proteins proteomes of beef. Of these, 92 had significant chan...

drought and salinity stress are adverse environmental factors that affect crop growth and yield. abiotic stress cause some reaction in plants such as change in gene expression, cell metabolism, growth and yield. moreover, stress condition resulted in increase of reactive oxygen species and disturbe macromolecules such as proteins, fatty acids, nucleic acid and pigments. in order to evaluate sal...

UNLABELLED Backgroud: Diabetic nephropathy is one of the most frequent causes of end-stage renal disease and is associated with proliferation of glomerular mesangial cells (MCs) and excessive production of the extracellular matrix (ECM). Several studies have shown that early growth response factor 1 (Egr1) plays a key role in renal fibrosis by regulating the expression of genes encoding ECM com...