The conjugated polymer, poly[3-(3-N,N-diethylaminopropoxy)thiophene] (PDAOT), was synthesized and employed in the supramolecular functionalization of single-walled carbon nanotubes (SWNTs). Highly stable aqueous solutions of PDAOT-SWNT complexes were obtained after sonication under mildly acidic conditions. UV-vis absorption and Raman spectroscopy studies showed that the noncovalent functionali...

BACKGROUND Oxygen deficiency in tumor tissue is associated with a malign phenotype, characterized by high invasiveness, increased metastatic potential and poor prognosis. Hypoxia chambers are the established standard model for in vitro studies on tumor hypoxia. An enzymatic hypoxia system (GOX/CAT) based on the use of glucose oxidase (GOX) and catalase (CAT) that allows induction of stable hypo...

In this paper, we have presented the use of flexographic printing techniques in the selective patterning of gold nanoparticles (AuNPs) onto a substrate. Highly uniform coverage of AuNPs was selectively patterned on the substrate surface, which was subsequently used in the development of a glucose sensor. These AuNPs provide a biocompatible site for the attachment of enzymes and offer high sensi...

Polystyrene(40)-b-poly(isocyanoalanine(2-thiophen-3-yl-ethyl)amide)(50) (PS-PIAT) polymersomes have the unique property of being sufficiently porous to allow diffusion of small (organic) substrates while retaining large biomolecules such as enzymes inside. Herein we report on the encapsulation and protection of glucose oxidase (GOx) and horse radish peroxidase (HRP) in PS-PIAT polymersomes and ...

Glucose and lactate enzyme electrodes have been fabricated through the deposition of an anionic self-assembled monolayer and subsequent redox polymer/enzyme electrostatic complexation on gold substrates. These surfaces were functionalized with a negative charge using 11-mercaptoundecanoic acid (MUA), followed by alternating immersions in cationic redox polymer solutions and anionic glucose oxid...

The development of new high-power biofuel cells has been limited in the past by slow or indirect charge transfer. In this study, enzymatic cell (EBFC) systems were explored with different materials used to evaluate their applicability as redox mediators. Redox mediators natures have selected for research. Cytochrome c, Chlorophyll a, and supernatant ultrasonically disrupted algae Chlorella vulg...

A new amperometric biosensor for copper determination in food samples was developed through the immobilization of glucose oxidase (GOx) in polyacrylamide gel. The response time for the reaction was studied by observing the percentage inhibition with time at 0.0125 ppm concentration of Cu 2+ . 2.30 minutes was the optimized response time for the estimation of Cu 2+ ion in the solution. Linear ra...

Glycolate oxidase (GOX) is a crucial enzyme of plant photorespiration. The encoding gene is thought to have originated from endosymbiotic gene transfer between the eukaryotic host and the cyanobacterial endosymbiont at the base of plantae. However, animals also possess GOX activities. Plant and animal GOX belong to the gene family of (L)-2-hydroxyacid-oxidases ((L)-2-HAOX). We find that all (L)...

The complex structure of glucose oxidase (GOX) with the substrate glucose was determined using a docking algorithm and subsequent molecular dynamics simulations. Semiempirical quantum chemical calculations were used to investigate the role of the enzyme and FAD co-enzyme in the catalytic oxidation of glucose. On the basis of a small active site model, substrate binding residues were determined ...

In the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly(vinyl alcohol) (PVA-COOH) to glucose oxidase (GOx) followed by the bioconjugation with CdS quantum-dots (QD). The resulting organic-inorganic nanohybrids were characterized by UV-visible spectroscopy, infrared spectroscopy, Photolumi...