The addition to the carbon ink, a major component of a screen-printed electrode (SPE), of an aliquot (10%) of Prussian Blue (PB)-modified glassy carbon (PB-GC) particles, resulted in an interference free “ready to use” amperometric H2O2 sensor (Vapp = −50 mV) with a LOD of 3 × 10−7 mol/l and a sensitivity of 135 mA (mol l−1 cm2). A storage stability of up to 8 months and an operational stabilit...

We developed a simple high-throughput colorimetric assay to detect glucose based on the glucose oxidase (GOx)-catalysed enlargement of gold nanoparticles (AuNPs). Compared with the currently available glucose kit method, the AuNP-based assay provides higher clinical sensitivity at lower cost, indicating its great potential to be a powerful tool for clinical screening of glucose.

A sensitive micro method for the manual estimation of true glucose in 10 mul plasma by an enzymatic technique is described. Protein precipitation is avoided. The method is simple to perform, accurate and precise. Agreement with a glucose oxidase autoanalyzer method is excellent, even with haemolysed and icteric specimens.

By coupling the Fenton reaction with the glucose oxidase (GOD)-catalyzed reaction, we have developed a fluorometric method for the determination of glucose; the linear response range and the detection limit are 0.108-2.59 microg/ml and 0.0432 microg/ml, respectively. This approach is selective and has the advantages of using inexpensive reagents and avoiding interferences. Satisfactory results ...

Diabetes is a widely spread chronic disease affecting about 463 million people in 2019. It defined as hyperglycemia caused by either an impaired action or abnormal secretion of insulin. New diabetes medications based on glucose-sensitive delivery systems can offer better control blood glucose levels without the need to worry patient diet frequency exercising. Such are comprised moieties such ox...

THE DETERMINATION of glucose by the glucose oxidase-peroxidase system has been simplified by Hill and Kessler (2) with the use of an automated system first described by Skeggs (1). Wincy and Marks (3) have also developed a method using o-tolidine in the automated system. These procedures require a large quantity of sample and use of a dialyzer to separate the protein before color development. T...

Biocontrol agents generally do not perform well enough under field conditions to compete with chemical fungicides. We determined whether transgenic strain SJ3-4 of Trichoderma atroviride, which expresses the Aspergillus niger glucose oxidase-encoding gene, goxA, under a homologous chitinase (nag1) promoter had increased capabilities as a fungal biocontrol agent. The transgenic strain differed o...

For the first time, a glucose oxidase-functionalized bioconjugate was prepared and served as a new trace label through its direct electrochemistry and electrocatalysis in a sandwich-type electrochemical aptasensor for ultrasensitive detection of thrombin.

Mitochondria and NADPH oxidase are important sources of reactive oxygen species in particular the superoxide radical (ROS) in pancreatic islets. These molecules derived from molecular oxygen are involved in pancreatic β-cells signaling and control of insulin secretion. We examined the involvement of ROS produced through NADPH oxidase in the leucine- and/or glucose-induced insulin secretion by p...