1. The incorporation of [U-14C]glucose into several lipid components of lung and liver slices, and the activities of glucose 6-phosphate dehydrogenase (EC 1.1.1.49), 6-phosphogluconate dehydrogenase (EC 1.1.1.44), 'malic' enzyme (EC 1.1.1.40) and NADP-isocitrate dehydrogenase (EC 1.1. 1.42) of the cell cytosol were examined in normal, starved and re-fed rats. 2. Lipogenesis and the activities o...

6,7 -Dideoxy-alpha-D-gluco-heptose 7-phosphonic acid, the isosteric phosphonate analogue of glucose 6-phosphate, was synthesized in six steps from the readily available precursor benzyl 4,6-O-benzylidene-alpha-D-glucopyranoside. The analogue is a substrate for yeast glucose 6-phosphate dehydrogenase, showing Michaelis-Menten kinetics at pH7.5 and 8.0. At both pH values the Km values of the anal...

Glucose g-phosphate dehydrogenase was first described in yeast by Warburg and Christian (l), who showed that nicotinamide adenine dinucleotide phosphate was the specific coenzyme. The enzyme was later prepared from liver and again shown to be specific for the same coenzyme (2). Subsequent preparations (see Cheldelin (3)) from various microbial sources have included two which react with both nic...

We describe two improved methods for determination of erythrocyte glucose-6-phosphate dehydrogenase activity. One method is an “enzyme-linked” procedure in which an excess of 6-phosphogluconate dehydrogenase is used to produce two moles of NADPHfor each mole of glucose-6-phosphate oxidized. In the other method 2,3-diphosphoglycerate is used to inhibit the variable contribution of endogenous 6-p...

Glucose-6-phosphate dehydrogenase deficiency is a common hemolytic genetic disorder, particularly in the areas endemic to malaria. The risk of drug induced Glucose-6-phosphate dehydrogenase deficiency related hemolysis depends on a number of factors including the Glucose-6-phosphate dehydrogenase variant. Aims: To know the G6PD deficiency and identify its variants in drug induced hemolysis in m...

1. Measurements were made of the non-oxidative reactions of the pentose phosphate cycle in liver (transketolase, transaldolase, ribulose 5-phosphate epimerase and ribose 5-phosphate isomerase activities) in a variety of hormonal and nutritional conditions. In addition, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities were measured for comparison with the oxidati...

The endogenous synthesis of cholesterol in hepatocyte nodules, induced in male Wistar rats, by a single dose of the hepatocarcinogen diethylnitrosamine followed by a selection procedure, was investigated and was compared with that in surrounding and control tissue. In addition, the activity of enzymes related to carbohydrate metabolism (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehy...

Glucose g-phosphate dehydrogenase was first described in yeast by Warburg and Christian (l), who showed that nicotinamide adenine dinucleotide phosphate was the specific coenzyme. The enzyme was later prepared from liver and again shown to be specific for the same coenzyme (2). Subsequent preparations (see Cheldelin (3)) from various microbial sources have included two which react with both nic...

OBJECTIVE Increased glucose 6-phosphate dehydrogenase activity has been demonstrated in heart failure. This study sought to assess myocardial glucose 6-phosphate dehydrogenase activity in retraining of the subpulmonary ventricle of adult goats. METHODS Eighteen adult goats were divided into three groups: traditional (fixed banding), sham, and intermittent (adjustable banding, daily 12-hour sy...