While Pseudomonas putida KT2440 has great potential for biomass-converting processes, its inability to utilize the biomass abundant sugars xylose and galactose limited applications. In this study, we utilized Adaptive Laboratory Evolution (ALE) optimize engineered with heterologous expression of xylD encoding xylonate dehydratase from Caulobacter crescentus galETKM UDP-glucose 4-epimerase, gala...

The heterogeneous asparagine-linked sugar chains of bovine and human immunoglobulins G were separated into 12 components by reversed-phase high performance liquid chromatography, and their structures were determined by 1H NMR spectroscopy. Both immunoglobulin (Ig) G sources contained eight non-bisected biantennary complexes and four bisected biantennary complexes. In the non-bisected sugar chai...

BACKGROUND The terminal transferase uridyl nick end labelling (TUNEL) assay allows the easy demonstration of cell death as a result of apoptosis. However, when this assay is applied to liver tissue, the number of TUNEL positive cells is dependent on the time of incubation with proteinase K. AIM To test whether false positive results are the result of the release of endogenous endonucleases by...

Human serum albumin (HSA) is abundant in blood. HSA binds a wide range of drugs, metabolites, and nutrients. A glycated also potential diabetes biomarker. Recently, crystal structures glucose- fructose-bound have been reported. Both cyclic acyclic sugar forms are trapped Sudlow site I. Galactose can bind HSA, but no atomic detail available. Thus, molecular dynamics simulations were employed to ...

The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscopy as [6Galfbeta1-6GalNAcbeta1-3Galalpha1-2ribitol-5-PO(4)(-)-6Galfbeta1-3Galbe...

Mitochondrial oxidative phosphorylation defects underlie many neurological and neuromuscular diseases. Patients’ primary dermal fibroblasts are one of the most commonly used in vitro models to study mitochondrial pathologies. However, tend rely more on glycolysis than for their energy when cultivated standard high-glucose medium, rendering it difficult expose dysfunctions. This aimed systematic...

Gracilariopsis lemaneiformis polysaccharide (GP) exerted protective effects in an aging mouse model administered with D-galactose, and its mechanism was revealed. GP increased the activity of antioxidant enzymes, reduced malondialdehyde levels brain serum, alleviated D-galactose–induced damage to liver, ameliorated mild cognitive impairment. Abundance gut microbiota differed significantly betwe...

The Sindbis virus glycoproteins, E l and E2, comprise a useful model system for evaluating the effects of local protein structure on the processing of N-linked oligosaccharides by Golgi enzymes. The conversion of oligomannose to N-acetyllactosamine (complex) oligosaccharides is hindered to different extents at the four glycosylation sites, so that the complex/oligomannose ratio decreases in the...