In this paper we demonstrate that maturing neoplastic cells from patients with chronic myelogenous leukemia (CML) constitutively produce G-CSF and are also receptive for this molecule. G-CSF functions as an autocrine growth factor in stable phase CML, and thus is responsible for divisions of maturing leukemic cells leading to an expansion of the compartment of mature cells. This observation is ...

Accumulation of bioactive compounds, during developmental stages of Graptopetalum paraguayense E. Walther, was investigated between 30 and 90days as a function of physiological maturity. Three distinct phases were defined: immature phase (30days), intermediate developmental phase (30-60days), and maturation phase (60-90days). Gallic acid and quercetin, antioxidative bioactive compounds, were id...

In this work we demonstrate measurements with optical coherence tomography (OCT) of the scattering phase function in the backward direction and the scattering anisotropy parameter g. Measurements of the OCT attenuation coefficient and the backscattering amplitude are performed on calibrated polystyrene microspheres with a time-domain OCT system. From these measurements the phase function in the...

Viruses can modify conditions inside cells to make them more favorable for replication and progeny virus production. One way of doing this is through manipulation of the cell cycle, a process that describes the ordered growth and division of cells. Analysis of model cell lines, such as A549 cells and primary airway epithelial cells, infected with human respiratory syncytial virus (HRSV) has sho...

To identify cell cycle-specific ultrastructural markers in acute lymphoblastic leukemia, we labeled bone marrow lymphoblasts with [3H]thymidine, separated them according to cell size by velocity sedimentation at 1 x g, and then identified the pattern of changes within the nucleus of the cells according to phase of the cell cycle. The smallest and most slowly sedimenting cells (probably in G,, p...

Nelarabine, prodrug of arabinosylguanine (ara-G), has demonstrated T-lymphoblastic antileukemic activity in cell lines and in the clinic. To investigate the mechanism for lineage-specific toxicity, the effects of ara-G were compared in CEM (T-lymphoblast), Raji (B-lymphoblast), and ML-1 (myeloid) cell lines. CEM cells were the most sensitive to ara-G-induced apoptosis and accumulated the highes...

Cytokine-mobilized peripheral blood hematopoietic stem cells (MPB HSC) are widely used for transplantation in the treatment of malignancies, but the mechanism of HSC mobilization is unclear. Although many HSC in bone marrow (BM) cycle rapidly and expand their numbers in response to cytoreductive agents, such as cyclophosphamide (CY), and cytokines, such as granulocyte colony-stimulating factor ...

chamomile ( matricaria chamomilla l.) is a widely used medicinal plant possessing several pharmacological effects due to presence of active compounds. this study describes a method of using ultra performance liquid chromatography (uplc) coupled with photodiode array (pda) detector for the separation of phenolic compounds in m. chamomilla and its crude extracts. separation was conducted on c 18 ...

BACKGROUND Control of the onset of DNA synthesis in mammalian cells requires the coordinated assembly and activation of the pre-Replication Complex. In order to understand the regulatory events controlling preRC dynamics, we have investigated how the timing of preRC assembly relates temporally to other biochemical events governing progress into S-phase. METHODOLOGY/PRINCIPAL FINDING In murine...