We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation. Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day. We show that EMP cloning has a higher efficiency than restriction-free (RF) cloning, especially for long inserts above 2.5 kb. EMP furth...

The minimum Se requirement for male turkey poults is 0.3 μg Se/g--three times higher than requirements found in rodents--based on liver and gizzard glutathione peroxidase-4 (GPX4) and GPX1 activities. In addition, turkey liver GPX4 activity is 10-fold higher and GPX1 activity is 10-fold lower than in rats, and both GPX1 and GPX4 mRNA levels are dramatically down-regulated by Se deficiency. Curr...

Can the optimum animal be defined? Will that definition change over time, by location, by market demand? First, assume what may be impossible, that the perfect animal can be defined or that only a limited number of definitions of "perfect" are needed. Then, can the "perfect" animal to match a definition be found? Suppose such an animal is found. Then the question to be answered before trying to...

UNLABELLED This research focused on understanding the attitudes toward human cloning in Taiwan among professionals in healthcare, law, and religion. DESIGN The study was conducted utilizing a structured questionnaire. PARTICIPANTS 220 healthcare professionals from two regional hospitals located in Taipei, 351 religious professionals in the northern Taiwan and 711 legal professionals were se...

Abstract At present, many cloning selection algorithms have been studied, and improvements made to the cloning, mutation steps. However, there is a lack of research on optimization updating operation The clonal algorithm traditionally updated through random complement antibodies, which blind uncertain process. added antibodies may gather near local optimal solution, resulting in need for more i...