Infectious Pancreatic Necrosis Virus (IPNV) is a member of the family Birnaviridae that has been linked to high mortalities in salmonids. Bacterial based systems as live vectors for the delivery of heterologous antigens offer a number of advantages as vaccination strategies. VP2 is a structural viral protein of IPNV with immunogenicity effects. In this study IPNV was isolated from diseased fry ...

Pulmonary arterial hypertension is characterized by pulmonary vascular remodeling, cellular proliferation, and poor long-term outcomes. Dysfunctional bone morphogenetic protein pathway signaling associated with both hereditary idiopathic subtypes. Sotatercept, a novel fusion protein, binds activins growth differentiation factors in the attempt to restore balance between growth-promoting growth-...

Background: Enterotoxigenic Escherichia coli (ETEC) strains are the major causes of diarrheal disease in humans and animals. Colonization factors and enterotoxins are the major virulence factors in ETEC pathogenesis. For the broad-spectrum protection against ETEC, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate. Methods: A fusion protein is composed of a...

Infectious Pancreatic Necrosis Virus (IPNV) is a member of the family Birnaviridae that has been linked to high mortalities in salmonids. Bacterial based systems as live vectors for the delivery of heterologous antigens offer a number of advantages as vaccination strategies. VP2 is a structural viral protein of IPNV with immunogenicity effects. In this study IPNV was isolated from diseased fry ...

In order to achieve optimal biological activity and desired pharmacokinetic profiles, a dithiocyclopeptide linker was designed for in vivo release of protein domains from a recombinant fusion protein. This novel in vivo cleavable disulfide linker, based on a dithiocyclopeptide containing a thrombin-sensitive sequence and an intramolecular disulfide bond, was inserted between transferrin and gra...

OBJECTIVES Staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. Antibiotic therapy for vancomycin resistant S. aureus (VRSA) can not promise the eradication of infections. Since adhesion is the major route of infections, adhesin based vaccine could suppress S. aureus infections. Fibronectin binding protein A (FnBPA) and clumping factor A (ClfA) ar...

Fusion protein systems can facilitate the purification of proteins produced by recombinant DNA technology (1-3). In these systems, a tag is fused to a target protein. The affinity of the tag for a specific ligand is exploited for the purification of the fusion protein. Here, we demonstrate that during affinity purification many truncated polypeptides carrying the affinity tag are copurified wit...

Photosynthetic Cyanobacteria can be used as a chassis for different synthetic biology approaches. However, quantitative comparison of tools engineering, such those heterologous gene expression, is often not available. Here, we report the comparative quantification protein production in Synechococcussp.PCC 7002 regarding expression cassettes and locations foreign integration using sf-GFP reporte...

The green fluorescent protein (GFP) of Aequorea victoria is frequently fused to other proteins to serve as a reporter for gene expression or the localization of proteins in vivo. We report that when a GFP fusion protein is translated in vitro under standard conditions, the GFP portion folds efficiently and becomes fluorescent. This provides a convenient method for monitoring in vitro translatio...

We have introduced amino acid substitutions into two regions of the extracellular domain of the vesicular stomatitis virus (VSV) glycoprotein (G protein) and examined the effect of these mutations on protein transport, low-pH-induced stability of G protein oligomers, and membrane fusion activity. We suggested previously that the region between amino acids 118 and 139 may be important for the me...