Cryo banking of spermatozoa is an essential aspect of fertility preservation. With the advancements in cryobiology and better understanding of cryoprotectants and assisted reproduction, indications for semen banking are expanding. The exponential developments that have occurred over the years in the field of cryopreservation have proved that frozen sperm is as good as fresh sperm in fertilizing...

The repeatability of the analysis of frozen thawed equine semen using a computer-assisted sperm analysis system is high. However, the settings used to analyze the semen samples influence the results to a large extent. Therefore, settings used in different studies should be described clearly, facilitating comparison of results. Authors’ address: Department of Reproduction, Obstetrics and Herd He...

The aim of this study was to compare the effect of different packaging systems on some parameters of cryopreserved canine spermatozoa. The experimental material consisted of the sperm-rich fractions of ejaculates collected from four Beagle dogs. Semen samples for cryopreservation were stored in 0.25 ml plastic straws and two aluminum tubes with a total volume of 5.0 ml. Semen was frozen in stat...

Ten ejaculates were used to test effects of slow (37.0 rain), moderate (7.8 rain) or fast (4.2 min) freezing from +5 to 1 2 0 C on post-thaw survival of sperm. Semen was extended at 37 C in Tris-yolk-glycerol, packaged i n . 3-ml Continental straws at ambient temperature, frozen at one of the three rates and thawed in 95 C water for 7 seconds. For combined post-thaw incubations of O, 3 and 6 hr...

Nine ejaculates were extended, placed in .5ml French straws and frozen vertically in two automatic forced vapor freezers (Linde BF-4 and FC-3) at three loads (2,500, 5,000 and full capacity of 7,500 straws in 5-straw goblets on canes). For each load, six locations within each freezing chamber were compared: upper and lower levels of the center, intermediate and corner areas. Semen was extended ...

Aim: The study was undertaken to quantify and to analyze the changes in the motion characteristics of bull spermatozoa during various stages of cryopreservation cycle. Materials and Methods: Using computer assisted semen analysis (CASA) technique, 26 ejaculates, collected from two Jersey bulls were analyzed for motility, head behaviour and swimming pattern of spermatozoa on dilution, pre-freeze...

The first report of a foal born after artificial insemination (AI) with frozen/thawed semen, more than fifty years ago, was the result of insemination with epididymal spermatozoa (Barker en Gandier, 1957). Ever since, reports have confirmed pregnancies and/ or births after AI with epididymal semen, which proves the fertilizing capacity of epididymal semen (Morris, 2002; Monteiro et al., 2011). ...

Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10 spermato...

Aim: The aim was to determine the antioxidative capacity of vitamin E, vitamin C and their combination (vitamin E+C) on standard semen parameters i.e., mortality, percent live sperms, percent abnormal sperms and acrosomal integrity in cryopreserved Bhadawari bull semen. Materials and Methods: Ejaculates collected from four Bhadawari bulls were evaluated and later pooled at 37°C. Pooled semen sa...

This study was carried out to identify the suitable buffer for cryopreservation of buffalo semen. Semen was collected with artificial vagina (42 degrees C) from four buffalo bulls. Split pooled ejaculates (n=5), possessing more than 60% visual sperm motility, were extended at 37 degrees C either in tri-sodium citrate (CITRATE), Tris-citric acid (TCA), Tris-Tes (TEST) or Tris-Hepes (HEPEST). Sem...