1. Broughton, P. M. G., A rapid ultraviolet spectrophotometric method for the detection, estimation and identification of barbiturates in biobogicab material. Biochem. J. 63, 207 (1956). 2. Block, W. D., Jarrett, J. K., Jr., and Levine, J. B., An improved automated determination of serum total chobesterob with a single cobor reagent. Clin. Chem. 12, 681 (1966). 3. Mattingly, D., A simple fluoro...

We report a continuous-flow fluorometric method for total urinary estrogens that involves the Kober reaction, with extraction of the reaction product into dichloroethane containing trichloroacetic acid as described by Hahnel and Jones [Clin. Chim. Acta 16, 185 (1967)]. The dichloroethane extraction gives greater stability to the Kober color and sharper separation of aqueous and organic phases. ...

Several different types of assay procedure have been proposed for the quantitative determination of thiamine. In a discussion (1) of the difficulties encountered in some of the methods, it has been pointed out that calorimetric (2, 3) and fluorometric (4) methods often suffer from a lack of sufficient sensitivity or specificity. The procedure described in the present communication, while not so...

With 4-chloro-7-nitrobenzo-2, 1,3-oxadiazole (I), fluorescent derivatives of propoxyphene can be prepared, thus enabling quantitation of the drug in biological samples. This technique offers an acceptable alternative to gas chromatographic (1-5) or ultraviolet spectrophotometric (6, 7) procedures. Favorable aspects of the fluorometric technique are high sensitivity, specificity, potential for r...