نتایج جستجو برای: fluorescent in situ hybridization

تعداد نتایج: 16994172  

Journal: :Environmental microbiology reports 2010
Junji Matsuo Kouhei Kawaguchi Shinji Nakamura Yasuhiro Hayashi Mitsutaka Yoshida Kaori Takahashi Yoshihiko Mizutani Takashi Yao Hiroyuki Yamaguchi

Obligate intracellular bacteria are commonly found as endosymbionts of acanthamoebae; however, their survival in and ability to transfer to amoebae are currently uncharacterized. In this study, six bacterial endosymbionts, found in five environmental Acanthamoeba isolates (S13, R18, S23, S31, S40) from different locations of Sapporo city, Japan, were characterized. Phylogenetic analysis reveale...

2017
Diego Javier Grassi Ana Claudia Swarça Jorge Abdala Dergam María Cristina Pastori Alberto Sergio Fenocchio

Hoplias malabaricus (Bloch, 1794), a predatory freshwater fish with a wide distribution throughout South America, represents a species complex with seven well characterized karyomorphs at the cytogenetic level. Although this species has been extensively studied in several Brazilian basins, data are still scarce for hydrographic systems from other South American countries. This study aims to cha...

2015
Roberto Ferreira Artoni Jonathan Pena Castro Uedson Pereira Jacobina Paulo Augusto Lima-Filho Gideão Wagner Werneck Félix da Costa Wagner Franco Molina

Fish constitute a paraphyletic and profusely diversified group that has historically puzzled ichthyologists. Hard efforts are necessary to better understand this group, due to its extensive diversity. New species are often identified and it leads to questions about their phylogenetic aspects. Cytogenetics is becoming an important biodiversity-detection tool also used to measure biodiversity evo...

Journal: :Human molecular genetics 1996
S L Page J C Shin J Y Han K H Choo L G Shaffer

Robertsonian translocations are the most common chromosomal rearrangements in humans. The vast majority of the ten possible nonhomologous types of Robertsonian translocations ascertained are rob(13q14q) and rob(14q21q). Recombination between homologous sequences on nonhomologous chromosomes has been proposed as a mechanism leading to the preferential formation of rob(13q14q) and rob(14q21q). Ho...

Journal: :Cytogenetics and cell genetics 1996
S Malaney H H Heng L C Tsui X M Shi B H Robinson

We have localized the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to chromosome 8 using both radioactive in situ hybridization and fluorescence in situ hybridization. The additional peak obtained with the former method is attributed to the higher sensitivity of this technique, which results in hybridization of the probe to the less conserved pseudogene. We ther...

Journal: :FEMS microbiology letters 2000
C Mercier E Domakova J Tremblay S Kulakauskas

In bacterial communities one bacterium can influence the growth of other members of the population. These interactions may be based on nutritional factors or may occur via bacterial signaling molecules that are released in the medium. We present an example, showing that in addition to the above means of interactions, muramidases, enzymes that specifically cleave peptidoglycan chains, can also m...

2017
Tianwei Zhang Lei Lv Yun Huang Xiaohui Ren Qinghua Shi

Asbestos is a well-known occupational carcinogen that can cause aneuploidy during the early stages of neoplastic development. To explore the origins of asbestos-induced aneuploidy, we performed long-term live-cell imaging followed by fluorescence in situ hybridization of chromosomes 8 and 12 in human bronchial epithelial (HBEC) and mesothelial (MeT5A) cells. We demonstrate that asbestos induces...

2016
Sarosh N. Fatakia Ishita S. Mehta Basuthkar J. Rao

Forty-six chromosome territories (CTs) are positioned uniquely in human interphase nuclei, wherein each of their positions can range from the centre of the nucleus to its periphery. A non-empirical basis for their non-random arrangement remains unreported. Here, we derive a suprachromosomal basis of that overall arrangement (which we refer to as a CT constellation), and report a hierarchical na...

Journal: :Journal of applied genetics 2005
Maria Lassota Barbara Przełozna Marta Płodzien Monika Bugno Maciej Wnuk Zbigniew Kotylak Ewa Słota

In this case report we present a child with an additional chromosome in the karyotype. The karyotypes of the boy and his parents were analyzed by use of a conventional banding technique (GTG) and fluorescence in situ hybridization (FISH). Probes painting whole chromosomes 12 and 18 were used in FISH. Cytogenetic examination of the parents revealed that his mother was carrying balanced reciproca...

Journal: :Genes & development 2014
Iain Williamson Soizik Berlivet Ragnhild Eskeland Shelagh Boyle Robert S Illingworth Denis Paquette Josée Dostie Wendy A Bickmore

Although important for gene regulation, most studies of genome organization use either fluorescence in situ hybridization (FISH) or chromosome conformation capture (3C) methods. FISH directly visualizes the spatial relationship of sequences but is usually applied to a few loci at a time. The frequency at which sequences are ligated together by formaldehyde cross-linking can be measured genome-w...

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