Abstract A liquid scintillator consisting of linear alkylbenzene as the solvent and 2,5-diphenyloxazole fluor was developed for SNO+ experiment. This mixture chosen it is compatible with acrylic has a competitive light yield to pre-existing scintillators while conferring other advantages including longer attenuation lengths, superior safety characteristics, chemical simplicity, ease handling, l...

Herein, we demonstrate the feasibility of a protein-protein interaction analysis and reaction progress monitoring in microfluidic droplets using FRET and microscopic fluorescence lifetime measurements. The fabrication of microdroplet chips using soft- and photolithographic techniques is demonstrated and the resulting chips reliably generate microdroplets of 630 pL and 6.71 nL at frequencies of ...

The first fluoride varnishes were developed during the 1960s (Duraphat) sodium fluoride varnish) and 1970s (Fluor Protector) silane fluoride varnish) to prolong the contact time between fluoride and enamel. Fluoride varnishes adhere to enamel, and calcium fluoride formed after application acts as a long-term reservoir of fluoride. Currently Duraphat varnish is the most widely used topical fluor...

Fluorescently labeled actin (Figure 11.1) is an important tool for investigating cytoskeleton dynamics in vivo.1–5 Molecular Probes offers highly purified actin from rabbit muscle (A-12375), as well as fluorescent actin conjugates labeled with two of our brightest and most photostable dyes. The green-fluorescent Alexa Fluor 488 actin conjugate (A-12373) has excitation and emission maxima simila...

Aberrant glycosylation during carcinogenesis results in altered glycan expression on oral cancer cells. The objective of this study was to detect this atypical glycosylation via imaging of fluorophore-conjugated lectins. Paired normal and tumor tissue from seven patients with oral squamous cell carcinoma were investigated for sialic acid expression via the legume protein wheat germ agglutinin (...

SYSTEMS AND METHODS Experiment Design and Rationale The data set was designed to provide a range of difficulty for pattern matching and detection of expression changes. mRNA samples A through T from 20 different cell lines and tissues listed in Table S1 were combined in different ways to provide condition pairs with both subtle and moderate expected expression differences. Figure S1 summarizes ...

A new synthesis approach is described that allows the direct incorporation of fluorescent labels into the volume or body of SiO2 nanoparticles. In this process, fluorescent Alexa Fluor dyes with different emission wavelengths were covalently incorporated into the SiO2 nanoparticles during their formation by the hydrolysis of tetraethoxysilane. The dye molecules were homogeneously distributed th...

Some protozoans are able to encyst as a protective response to a harmful environment. The cyst wall usually contains chitin as its main structural constituent. Acanthamoeba is an exception since its cyst wall contains cellulose. Specific cytochemical differentiation between cellulose and chitin by microscopy has not been possible due to the similarity of the constituent beta-1,4-linked hexose b...

Partially folded protein species transiently form during folding of most proteins. Often, these species are molten globules, which may be on- or off-pathway to the native state. Molten globules are ensembles of interconverting protein conformers that have a substantial amount of secondary structure, but lack virtually all tertiary side-chain packing characteristics of natively folded proteins. ...