OBJECTIVE G-Banding followed by standard chromosome analysis is routinely used for prenatal detection of chromosomal abnormalities. In recent years, molecular cytogenetic techniques have been developed for rapid diagnosis of chromosomal abnormalities. Among these methods Quantitative Florescence Polymerase Chain Reaction (QF-PCR) has been widely used for this purpose. Heterozygosity of short ta...

Hyperacute kidney rejection is unusual in crossmatch positive recipients of simultaneous liver-kidney transplants (SLKT). However, recent data suggest that these patients remain at risk for antibody-mediated kidney rejection. To further investigate the risk associated with donor-specific alloantibodies (DSA) in SLKT, we studied 86 consecutive SLKT patients with an available pre-SLKT serum sampl...

Tissue samples (plasma, saliva, serum or urine) from 169 patients classified as either normal or having one of seven possible diseases are analysed across three 96-well plates for the presences of 37 analytes using cytokine inflammation multiplexed immunoassay panels. Censoring for concentration data caused problems for analysis of the low abundant analytes. Using fluorescence analysis over con...

Chemical fungicides viz. propiconazole, thiram, bayleton, copper-oxy-chloride, benomyl and mancozeb in three different concentrations were screened for their fungicidal efficacy against P. pinnata seed mycoflora viz., Penicillium sp., A. oryzae, A. ustus, A. sydowii, A. fumigatus, A. flavus, A. niger, Rhizopus sp., Fusarium sp., C. gloeosporioides and Absidia sp. in in-vitro conditions by poiso...

Among several strategies used for Herpes simplex virus (HSV) detection in biological specimens, standard plaque assay (SPA) remains the most reliable method to evaluate virus infectivity and quantify viral replication. However, it is a manual procedure, thereby affected by operator subjectivity, and it may be particularly laborious for multiple sample analysis. Here we describe an innovative me...

Wheat grain protein content (GPC) is a key component when evaluating wheat nutrition. It is also important to determine wheat GPC before harvest for agricultural and food process enterprises in order to optimize the wheat grading process. Wheat GPC across a field is spatially variable due to the inherent variability of soil properties and position in the landscape. The objectives of this field ...

The fourth EMBO-sponsored conference on Experimental Approaches to Evolution and Ecology Using Yeast and Other Model Systems (https://www.embl.de/training/events/2016/EAE16-01/), was held at the EMBL in Heidelberg, Germany, October 19-23, 2016. The conference was organized by Judith Berman (Tel Aviv University), Maitreya Dunham (University of Washington), Jun-Yi Leu (Academia Sinica), and Lars ...

Mixed gliomas, primarily oligoastrocytomas, account for about 5%-10% of all gliomas. Distinguishing oligoastrocytoma based on histological features alone has limitations in predicting the exact biological behavior, necessitating ancillary markers for greater specificity. In this case report, human telomerase reverse transcriptase (hTERT) and high mobility group-A1 (HMGA1); markers of proliferat...

The treatment of cancer draws interest from researchers worldwide. Of the different extracts from traditional Chinese medicines, Tubeimoside 1 (TBMS 1) is regarded as an effective treatment for cancer. To determine the mechanism of TBMS 1, the shape/pattern of HepG2 cells based on the microscopic imaging technology was determined to analyze experimental results; then the fluorescent spectra met...

Objectives: The purpose of this study is to explore the long-range effects of the Reconnective Healing upon vegetative regulation of the heart rhythm, energy reserve parameters and immune status in experimental groups compared with control cohorts. Methods: The Heart Rate Variability (HRV), Electrophotonic Imaging (Gas Discharge Visualization EPI/GDV) camera and blood analysis were used to asse...