conclusions these results indicated that eaec strains isolated from iranian children with diarrhea were heterogeneous and showed high resistance rates against commonly used antibiotics, which was similar to that reported in studies performed in other countries. results significant percentage of local eaec strains carried the virulence plasmid genes and formed biofilms. in addition, these strain...

conclusions with regard to improving the understanding of the differences in the biodiversity of the fecal microbiota between rd infants and h infants, the findings of this study can provide a possible basis to reveal the relationship between rd and intestinal microbiota. objectives this study aimed to reveal the changes in the biodiversity of the fecal microbiota of infants with rd. patients a...

the edible mushrooms (basidomycetes) have high nutritional value, promote the immune system, and as a source of natural antimicrobial substances have been used to cure bacterial infections since ancient times.various kinds of proteins with several biological activities are produced by mushrooms. in this research, in order to evaluate antibacterial activity of edible mushrooms, we isolated prote...

Four of the membrane proteins from Acholeplasma laidlawii that are soluble in the nonionic detergent Tween 20 have been purified by preparative electrophoretic techniques utilizing different supporting media. The last purification step for two of the major proteins was a preparative polyacrylamide gel electrophoresis performed in the absence of any detergent. The proteins were recovered by cont...

Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors’ lab in this domain and highlights the capabilities and limitations of gel electrophoresis. Pitfalls of the technique were recognized by identifying the origin of...

In this study, we investigated the role of ovalbumin (OVA), ovotransferrin (OVT), and lysozyme (LYZ) as major proteins involved in heat-induced gelation egg white. OVT LYZ deficient white (OLdEW) solutions were prepared from raw (REW) subjected to heat treatment at 64 °C centrifugation. OLdEW had almost same native-OVA protein REW, confirmed by gel electrophoresis, differential scanning calorim...

An amphiphilic low-molecular-weight hydrogelator 1 was synthesized. A tris-glycine-SDS solution gel of 1 was applied for electrophoresis to separate proteins. Centrifugation of a mixture of protein and a hydrogel of 1 enabled the recovery of protein. Various combinations of proteins were applied for supramolecular gel electrophoresis (SUGE), and remarkably poor mobility for small proteins (<45 ...

A versatile system (PHOGE) has been developed that allows resolution of molecules of DNA megabase pair size by the use of homogeneous, orthogonal, pulsed fields. The resulting electrophoretograms have characteristics that differ from those produced by other systems for pulsed field electrophoresis. Molecules in a two-fold range of sizes can be separated with maximum resolution, or a much larger...