Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized heavy chains (HC) bound by a light chain (LC). In addition to binding HC, LC interacts with members of the Hip1/R family of endocytic proteins, including the budding ye...

The aim of present study was to assess the effect of glucogenic (G) and lipogenic (L) diets on blood metabolites in Baloochi lambs. Three rumen cannulated Baloochi sheep were used as a 3×3 Latin square design with 3 periods (28 days). Experimental diets were a glucogenic, a lipogenic and a mixture of G and L diets (50:50). The animals were fed diets consisted of 50% chopped alfalfa hay and 50% ...

An experiment was conducted to compare the effect of corn-soybean meal finisher (29-42d) diets containing flaxseeds (rolled or extruded) on pellet quality, performance, n-3 fatty acids (FA) and oxidative stability of meat in broiler chickens. Seven pelleted diets were provided in a 3×2 factorial arrangement with three flaxseed levels (5, 10, and 15%) and two processing methods (rolled or extrud...

BACKGROUND AND AIMS Hepatitis C (HC) is a major cause of hepatocellular carcinoma (HCC), and a late diagnosis is the main factor for the poor survival of patients. There is an urgent need for identifying sensitive and specific biomarkers for HCC diagnosis. In the present study, plasma lipid patterns of patients with HC-HCC, HC-liver cirrhosis (LC), and chronic HC (CHC) were assessed by matrix-a...

Developing non-invasive alternatives to monitor insulin levels in humans holds potential practical value for identifying individuals with, or at risk of developing, insulin resistance. The aims of this study were: (1) to determine if saliva insulin can be used to delineate between low and high postprandial insulin levels following the ingestion of mixed breakfast meals; and (2) to determine if ...

Obesity is associated with impaired endothelial-dependent flow-mediated dilation, a precursor to hypertension and atherosclerosis. Although dieting generally improves cardiovascular risk factors, the direct effect of different dietary strategies on vascular endothelial function is not known. The purpose of this study was to test the hypothesis that a low-fat (LF) diet improves endothelial funct...

Two independent experiments were conducted with broilers to determine the effect of threonine and vitamin A on immune system. In first experiment, dietary treatments contained 0.8%, 0.87%, 0.94 % and 1.01 % total threonine. In second experiment, diets were as follows: basal diet without vitamin A; basal diet plus 1500 IU/Kg vitamin A; basal diet plus 6250 IU/Kg vitamin A; basal diet plus 11000 ...

It was hypothesized that a high-concentrate diet fed during early calfhood alters the expression of genes within the arcuate nucleus that subserve reproductive competence. Beef heifers (n = 12) were weaned at approximately 3 mo of age, and after acclimation, were allocated randomly to 1 of 2 nutritional groups: 1) High Concentrate/High Gain (HC/HG), a high concentrate diet fed to promote a gain...

Two independent experiments were conducted with broilers to determine the effect of threonine and vitamin A on immune system. In first experiment, dietary treatments contained 0.8%, 0.87%, 0.94 % and 1.01 % total threonine. In second experiment, diets were as follows: basal diet without vitamin A; basal diet plus 1500 IU/Kg vitamin A; basal diet plus 6250 IU/Kg vitamin A; basal diet plus 11000 ...

Nicotine replacement by transdermal patches is more effective than placebo in smoking cessation, but has a low success rate after one year (9-18%). We tested whether this was attributed to insufficient nicotine replacement. We conducted a randomized trial to investigate the effect on outcome of different doses of transdermal nicotine replacement after stratification according to baseline plasma...