First report on the production of protease through fed batch cultivation using co-culture of Pseudomonas putida and Staphylococcus aureus from a crude carbon source, banana peel. Batch production by using synthetic medium, impact of some of the process parameters on cell growth and alkaline protease activity was investigated and the optimized values were found to be inoculum size of 5 % (v/v), ...

Improved production costs will accelerate commercialization of polyhydroxyalkanoate (PHA) polymer and PHA-based products. Plant oils are considered favorable feedstocks, due to their high carbon content and relatively low price compared to sugars and other refined carbon feedstocks. Different PHA production strategies were compared using a recombinant strain of Ralstonia eutropha that produces ...

A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...

The rising costs of bioprocess research and development emphasize the need for high-throughput, low-cost alternatives to bench-scale bioreactors for process development. In particular, there is a need for platforms that can go beyond simple batch growth of the organism of interest to include more advanced monitoring, control, and operation schemes such as fed-batch or continuous. We have develo...

Fed-batch animal cell culture is the most common method for commercial production of recombinant proteins. However, higher cell densities in these platforms are still limited due to factors such as excessive ammonium production, lactic acid production, nutrient limitation, and/or hyperosmotic stress related to nutrient feeds and base additions to control pH. To partly overcome these factors, we...

Improved production costs will accelerate commercialization of polyhydroxyalkanoate (PHA) polymer and PHA-based products. Plant oils are considered favorable feedstocks, due to their high carbon content and relatively low price compared to sugars and other refined carbon feedstocks. Different PHA production strategies were compared using a recombinant strain of Ralstonia eutropha that produces ...

An unconventional modelling methodology based on artificial neural networks is proposed to rapidly develop a model from data obtained during different batch experiments. The objective of the global predict time evolution concentrations all species present in reaction medium. For this, recurrent are elaborated estimate particular as function operating parameters and then assembled complex model....

Glossary batch process A technique in which all nutrients for the microorganisms are added to the bioreactor only once and the products are separated in their entirety when the fermentation process is completed. bioreactor or fermenter The culture vessel or system used for growing microorganisms and producing microbial metabolic products such as lactic acid. continuous process A technique in wh...

The production of recombinant proteins in bioreactors requires real-time process monitoring and control to increase process efficiency and to meet the requirements for a comprehensive audit trail. The combination of optical near-infrared turbidity sensors and dielectric spectroscopy provides diverse system information because different measurement principles are exploited. We used this combinat...