There is great interest in mesenchymal stromal cells (MSCs) for cell-therapy and tissue engineering approaches. MSCs are currently expanded in vitro in the presence of fetal calf serum (FCS); however, FCS raises concerns when used in clinical grade preparations. The aim of this study was to evaluate whether MSCs expanded in medium supplemented with platelet-lysate (PL), already shown to promote...

One of the actions to reduce Greenhouse Emissions is decarbonization transport sector. Among alternatives for it, there are Electric Vehicles (EV). However, increase EV penetration, mainly in large countries, like Brazil or US, it necessary development Public Charging Infrastructure, such as Fast-Charging Stations (FCS) on Highways, handle long-distance trips without long-charging times. The hi...

BACKGROUND Amnestic mild cognitive impairment (aMCI), which is recently considered as a high risk status for developing Alzheimer's disease (AD), manifests with gray matter atrophy and increased focal functional activity in the medial temporal lobe (MTL). However, the abnormalities of whole-brain functional network connectivity in aMCI and its relationship to medial temporal atrophy (MTA) remai...

Different mammalian cells in culture have individual nutritional requirements, which are mainly fulfilled by the addition of foetal calf serum (FCS) to the basic medium. Collecting FCS is accompanied with severe animal welfare problems as conscient animals usually are bleeded to death by heart-punctuation without anaesthetics. There exists scientific problems too. Due to the batch-to-batch vari...

Oviductal factors may be obtained by ultrafiltration of conditioned medium, added to a simple media and used in bovine embryo culture. In this study, we aimed to analyze the development of bovine embryos produced with oviductal factors compared to those cultured in the presence of BSA or serum, the effects of glucose in presence of these protein supplements, and the ability of oviductal factors...

BACKGROUND Identification of von Willebrand factor (vWF) abnormalities in a variety of conditions is hampered by the limitations of currently available diagnostic tests. Although direct multimer visualization by immunoelectrophoresis is a commonly used method, it is impractical as a routine clinical test. In this study, we used a biophysical analysis tool, fluorescence correlation spectroscopy ...

We assess the novelty and maturity of software (SW) agent-based systems (ABS) for the Future Combat System (FCS) concept. The concept consists of troops, vehicles, communications, and weapon systems viewed as a “system of systems” [including net-centric command and control (C) capabilities]. In contrast to a centralized, or platformbased architecture, FCS avoids decision-making/execution bottle...

BACKGROUND Although several prior works reported that apathy is associated with conversion to Alzheimer's disease in individuals with amnestic mild cognitive impairment (aMCI), effects of apathy on the functional connectivity (FC) of the brain remain unclear. In this study, we assessed the pattern of association between apathy and default mode network (DMN), salience network and central executi...

We developed an organoid culture technique to study the mechanisms involved in arterial remodeling. Resistance arteries were isolated from rat cremaster muscle and mounted in a pressure myograph at 75 mmHg. Vessels were studied during a 4-day culture period in DMEM with either 2% albumin, 10% heat-inactivated FCS (HI-FCS) or 10% dialyzed HI-FCS (12 kDa cut off) added to the perfusate. The album...

Recently, there is an increasing research efforts in XML data mining. These efforts largely assumed that XML documents are static. However, in many real applications, XML data are evolutionary in nature. In this paper, we focus on mining evolution patterns from historical XML documents. Specifically, we propose a novel approach to discover frequently changing structures (FCS) from a sequence of...