Because of its pivotal role in the metabolism of circulating lipids, lipoprotein lipase has been the subject of concerted investigation for many years. Although much of the information that has been forthcoming has been reviewed in the recent past (Cryer, 1981 ; Quinn et al., 1982 ; Hamosh and Hamosh, 1983 ; Cryer, 1983) it is only fair to say that the ontogenic aspects of the enzyme and its ac...

Plasma phospholipid transfer protein (PLTP) is thought to play a major role in the facilitated transfer of phospholipids between lipoproteins and in the modulation of high density lipoprotein (HDL) particle size and composition. However, little has been reported concerning the relationships of PLTP with plasma lipoprotein parameters, lipolytic enzymes, body fat distribution, insulin, and glucos...

The role of the enzyme hepatic triglyceride lipase was investigated in a primate model, the cynomolgus monkey. Antisera produced against human postheparin hepatic lipase fully inhibited cynomolgus monkey posttheparin plasma hepatic triglyceride lipase activity. Lipoprotein lipase activity was not inhibited by this antisera. Hepatic triglyceride lipase activity in liver biopsies was decreased by...

ApoC-II was the only apolipoprotein from human very low density lipoprotein that activated rat adipose tissue lipoprotein lipase. Activation was blocked by antiserum against apoC-II. Addition of increasing amounts of activator did not alter the apparent Knl of lipoprotein lipase (0.32 mM triolein), but it did produce a progressive increase in the apparent Vm8V from 0.8 to 2.2 yxmoles free fatty...

Experiments were carried out to study the role played by the extrahepatic and hepatic lipolytic enzymes in lipoprotein catabolism. Chylomicra and very low density lipoproteins containing [I-“Hlglyceryl triacylglycerols radiolabeled in uiuo were incubated with purified milk lipoprotein lipase to produce lipoprotein remnants rich in monoacylglycerol. The primary products of the milk lipoprotein l...

To elucidate mechanisms responsible for the increased plasma triglyceride (TG) which occurs in diet-induced hypercholesterolemia in rabbits, albino rabbits were fed a 3% cholesterol diet for various durations, and the following determinations were carried out: plasma lipid levels; turnover rate of plasma TG (labeled very low density lipoprotein, or glycerol-2-H method); lipoprotein lipase activ...

Previously we demonstrated a marked sex difference in the metabolism of chylomicrons in adult rats. In males, radiolabeled chylomicrons displayed a longer dwell time on endothelial surfaces, which resulted in a decreased chylomicron uptake by the liver. The increased rate of chylomicron metabolism in females was associated with increased postheparin lipoprotein lipase activity. In the present s...

Lipoprotein lipase (LPL) is the rate-limiting enzyme for the hydrolysis of the triglyceride-rich lipoproteins and plays a critical role in lipoprotein and free fatty acid metabolism. Genetic manipulation of LPL may be beneficial in the treatment of hypertriglyceridemias, but it is unknown whether increased LPL activity may be effective in lowering plasma cholesterol and improving insulin resist...

Alimentary triglycerides are transported in the blood primarily in the form of emulsified particles called chylomicrons, which consist predominantly of triglycerides, but also contain a small proportion of cholesterol, phospholipid, and protein (1). Recently, Rodbell and Fredrickson (2) have found that one of the proteins associated with chylomicrons appears to be the same as the protein isolat...