The present study was aimed to construct a fusion plasmid harboring the extracellular domain of the influenza A M2-protein (M2e), which was fused to the N-terminus of the truncated HSP70 (HSP70359–610) molecule as a new approach for future vaccine research against influenza A. The amplified fragments, M2e and HSP70359-610 genes, were gel-purified. The products were then single digested with Bam...

Extracellular polysaccharides are synthesized by a wide variety of species, from unicellular bacteria and Archaea to the largest multicellular plants and animals in the biosphere. In every case, the biosynthesis of these polymers requires transport across a membrane, from the cytosol to either the lumen of secretory pathway organelles or directly into the extracellular space. Although some poly...

Botryococcus is a microalgal genus known for its ability to generate and accumulate substantial amounts of lipids via carbohydrate metabolism. This work determined the metabolic pathways enzymes involved in metabolism leading increased synthesis fat sp. Relevant intracellular extracellular metabolites were extracted quantified using chromatographic analysis. Enzymes lipid formation under natura...

Ectoine, a heterocyclic amino acid produced by various bacteria, was widely used in the fields of cosmetics and medicine. In this study, novel ectoine synthesis cluster from marine bacterium Salinicola salarius 1A01339 firstly introduced into Escherichia coli BL21(DE3) for heterologous production ectoine. The bioinformatic analysis proved function these enzymes, showed highest identities 83.3–8...