Potency is a key optimization parameter for hemophilia A gene therapy product candidates. Optimization strategies include promoter engineering to increase transcription, codon optimization of mRNA to improve translation, and amino-acid substitution to promote secretion. Herein, we describe both rational and empirical design approaches to the development of a minimally sized, highly potent AAV-f...

Installing a power system voltage regulator (PSVR) on the condenser can enhance dynamic control capability of local high-voltage bus voltage, but there are still few studies optimization PSVR parameters. The model is built to determine analytical expression between disturbance and change target, influence trend characteristics analyzed. Furthermore, in MATLAB/Simulink environment, PSVR-containi...

background: breast cancer is one of the most prevalent malignancies among women in various countries. her2 overexpression, which is due to different reasons, occurs in 20-30% of breast cancers. her2 gene encodes an 185kda transmembrane glycoprotein with 1255 amino acids. this active product triggers downstream intracellular signaling pathways inducing cell proliferation and cell survival. these...

BACKGROUND Escherichia coli phytase is an acidic histidine phytase with great specific activity. Pichia pastoris is a powerful system for the heterologous expression of active and soluble proteins which can express recombinant proteins in high cell density fermenter without loss of product yield and efficiently secrete heterologous proteins into the media. Recombinant protein expression is infl...

in recent years, due to absence of integrated watershed management in most regions, natural resources have witnessed numerous damages with severe floods as one of its consequences which further leads to economic, social and environmental damages. presently, there is no optimal utilization of land in most watersheds hence no optimal model matching with facilities and objectives is used in the wa...