Background: Aquatic ecosystems are an important source of nontuberculous mycobacteria (NTM) that can cause different diseases in human. Since culture of mycobacteria needs long-term incubation, fast-growing microorganisms and contaminants in the environment usually prevents the isolation of mycobacteria. Here, we compare different treatment protocols and describe a method that increases the rec...

Cut gerbera (Gerbera jamesonii) flowers are sensitive to microbial contamination and have short vase life. The effect of salicylic acid, citric acid and ascorbic acid (50, 100 and 200 mg l-1) was evaluated on vase life of gerbera flowers. Changes in vase life, water absorption, and bacterial population in stem and vase solution, also biochemical characteristics such as protein concentration, li...

Conservation and propagation of species using biotechnologic tools-such as plant tissue culture-are relevant when natural propagation is hampered for various reasons. In vitro techniques allow mass multiplication and propagation under pathogen-free conditions but also override dependence on season for availability of plant material. Moreover, in vitro genetic manipulation of a species, invariab...

Clavulanic acid is produced industrially by fermentation of Streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant DNA technology, and media composition and growth condition optimization. The main objective of this study was to increase the level of clavulanic acid production from Streptomyces clavuligerus (DSM 738), using UV irradiation. After...

in vitro propagation of plant has played a very important role in rapidmultiplication of cultivars with desirable traits and production of healthy and disease-free plants. in the present investigation, the objectives were to optimize the micropropagation of rose hybrid ‘black baccarat’ cultivar. in proliferation step, the nodal segments (1.5 cm) was cultured on both liquid and solid media (ms, ...

A reliable regeneration system for faba bean has been difficult to establish and therefore, the genetic improvement of Vicia faba L. was delayed. The paper describes a method of somatic embryo induction in callus of V. faba. Two Egyptian faba bean cultivars 'Giza 2' and '24 Hyto' were used. Callus was induced from epicotyls and shoot tips cultured on MS or Gamborg medium supplemented with 3% su...

Preliminary experiments have shown (1, 2) that, when excised tobacco leaves are cultured in the dark in 0.2 M solutions of potassium succinate or L-malate, a marked stimulation of the formation of citric acid occurs. Furthermore, leaves cultured in succinate increased in their content of malic acid, although culture in L-malate, in the single experiment that has been carried out (2), did not le...