The oxidation of formaldehyde by rat liver mitochondria in the presence of 50 mM phosphate was enhanced 2-fold by exogenous NAD+. Absolute requirement of NAD+ for formaldehyde oxidation was demonstrated by depleting the mitochondria of their NAD+ content (4.6 nmol/mg of protein), followed by reincorporation of the NAD+ into the depleted mitochondria. Aldehyde (formaldehyde) dehydrogenase activi...

The purpose of this on-farm trial was to evaluate the impact supplementing slow-release nitrogen (SRN; min. 40% N) and exogenous fibrolytic enzyme (EFE; cellulase, xylanase, glucanase, mannanase xylanase-potentiating factor) products on milk yield composition in dairy cows. Twelve early-to-midlactating crossbred cows were divided into 2 groups with comparable production. Animals fed a total mix...

Purification of cyclic 3’,5’-nucleotide phosphodiesterase of bovine brain cerebrum resulted in partial loss of activity, due to dissociation of an activator (or cofactor) from the enzyme. A systematic study showed that as purification proceeded, the activator was removed from phosphodiesterase in a stepwise fashion. Fractions representing phosphodiesterase at different stages of purification co...